Anaerobic microbial growth near thermodynamic equilibrium as a function of ATP/ADP cycle: The effect of maintenance energy requirements

Date

2013-12-01

Author

Şengör, Sema Sevinç
Brugato, Christopher J.
Gikas, Petros

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

28
views

0
downloads

For predicting microbial metabolism in low energy yielding environments, various rate laws have been proposed to account for the effects of thermodynamic state (as a measure of product-inhibition) as well as maintenance requirements on energetics of mediated reactions. Explicit or implicit modeling of simplified ATP reactions allows distinction between energy and ATP producing (catabolic, treated as kinetic and reversible) and energy and ATP consuming (anabolic) processes including maintenance requirements. Here, we provide a comparison of several approaches for modeling microbial metabolism in anaerobic environments considering thermodynamic factors, and maintenance energy requirements. We develop a mathematical model for microbial metabolism in anaerobic systems, which couples catabolic and anabolic processes considering the limiting effects of intermediate concentrations on reaction rate through the reduction of chemical potential and reversibility, and that explicitly partitions energy (ATP) allocation between cell growth and maintenance. We include an approach where maintenance energy requirements are assumed to take precedence over ATP-consuming cell synthesis reactions. Also, substrate utilization terminates when the catabolic reactions reach thermodynamic equilibrium with respect to ATP formation, including maintenance energy. The comparison of the proposed model to other modeling approaches shows the benefits of incorporating product inhibition and maintenance requirements in situations which maintenance energy requirements are comparable in size to growth energy requirements. An example application is also presented, where the proposed model is applied to an experimental study of arsenate reduction by Bacillus arsenicoselenatis conducted by Blum et al. (Arch Microbiol. 171 (1998) 19-30), in which the rate of metabolism is controlled by thermodynamics. (C) 2013 Elsevier B.V. All rights reserved.

URI

https://hdl.handle.net/11511/94017

Journal

BIOCHEMICAL ENGINEERING JOURNAL

DOI

https://doi.org/10.1016/j.bej.2013.10.006

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Anaerobic mesophilic co-digestion of sugar-beet processing wastewater and beet-pulp in batch reactors Alkaya, Emrah; Demirer, Göksel Niyazi (2011-03-01) In this study, biochemical methane potential (BMP) assay was conducted to investigate the effect of waste mixing and F/M ratio on the co-digestion of wastewater and beet-pulp, in addition to the digestion of the wastes separately. In the studied F/M range (0.51–2.56 g COD/g VSS), observed treatment efficiencies (63.7–87.3% COD removal and 69.6–89.3% VS reduction) were indications of high biodegradability for both wastewater and beet-pulp, which decreased with increasing F/M. It was evident that the extent o...
HYPERTHERMOPHILIC HYDROGEN PRODUCTION BY GEOGLOBUS ACETIVORANS IN MICROBIAL ELECTROLYSIS CELLS Kaş, Aykut; Yılmazel Tokel, Yasemin Dilşad; Department of Environmental Engineering (2021-9-10) Utilization of hyperthermophilic microorganisms was suggested to improve reaction rates and insoluble pollutant degradation and minimize the risk of contamination in bioelectrochemical systems (BESs). So far only a small group of hyperthermophilic microorganisms were identified, which show the ability to donate electrons to solid electrodes in BESs and here we present a new culture that fits to this description. The iron reducing archaeal culture Geoglobus acetivorans, originally isolated from a hydrotherma...
Anaerobic co-digestion of sewage sludge and primary clarifier skimmings for increased biogas production Alanya, S.; Yılmazel Tokel, Yasemin Dilşad; Park, C.; Willis, J. L.; Keaney, J.; Kohl, P. M.; Hunt, J. A.; Duran, M. (IWA Publishing, 2013-01-01) The objective of the study was to identify the impact of co-digesting clarifier skimmings on the overall methane generation from the treatment plant and additional energy value of the increased methane production. Biogas production from co-digesting clarifier skimmings and sewage sludge in pilot-scale fed-batch mesophilic anaerobic digesters has been evaluated. The digester was fed with increasing quantities of clarifier skimmings loads: 1.5, 2.6, 3.5 and 7.0 g COD equivalent/(L.d) (COD: chemical oxygen dem...
Anaerobic-Fed and Sequencing-Batch Treatment of Sugar-Beet Processing Wastes: A Comparative Study Alkaya, Emrah; Demirer, Göksel Niyazi (Wiley, 2011-03-01) The aim of this study was to compare a batch-fed continuously mixed anaerobic reactor (FCMR) with an anaerobic sequencing batch reactor (ASBR), in terms of waste stabilization and methane production treating sugar-beet processing wastewater and beet-pulp simultaneously. A reactor was operated as FCMR, which then was operated as an ASBR by changing operational conditions after the steady-state was reached. Although the hydraulic retention time value of the ASBR configuration was lower (8 days) than that of t...
Anaerobic treatment of dilute wastewaters Alptekin (Ener), Emel; Demirer, Göksel Niyazi; Department of Environmental Engineering (2008) In this study, domestic wastewater was used to determine the anaerobic treatment performances of a dilute wastewater in one-phase upflow anaerobic sludge blanket (UASB) system and two-phase upflow anaerobic filter (UAF) and UASB system. The acidification performances of domestic wastewater were compared in UAF and UASB reactors. The degree of acidification is higher in UAF reactor (31%) compared to UASB reactor (18%) at 2 h hydraulic retention time (HRT) in the anaerobic treatment of domestic wastewaters. T...

Citation Formats

S. S. Şengör, C. J. Brugato, and P. Gikas, “Anaerobic microbial growth near thermodynamic equilibrium as a function of ATP/ADP cycle: The effect of maintenance energy requirements,” BIOCHEMICAL ENGINEERING JOURNAL, vol. 81, pp. 65–72, 2013, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/94017.