Formaldehyde biosensing in air using fluorescent bacterial bioreporter cells

Elcin, Evrim
Ayaydin, Ferhan
Öktem, Hüseyin Avni
Formaldehyde is a genotoxic volatile organic pollutant and one of the causative agents of sick building syndrome. Despite of its hazardous carcinogenic effects, it has been still used in daily life products and household materials. Hence, determination of formaldehyde both in ambient air and drinking water sources is crucial to prevent its adverse health effects. Whole-cell biosensors have emerged as bio-sentinels for environmental monitoring to assess pollution in air, water and soil. Herein whole-cell bacterial bioreporter was developed based on a DNA damage response gene promoter and green fluorescent reporter protein, and the cells were entrapped in calcium-alginate hydrogel beads for sensitive detection of formaldehyde in air. Immobilisation enables portability, on-site detection ability and integration into mobile devices. Alginate bead-immobilised bioreporter could successfully detect formaldehyde in gas phase at concentration minimum of 8.1 ppm. This detection limit is useful for monitoring cumulative doses of bioavailable gaseous formaldehyde and taking precaution to avoid acute toxicity of formaldehyde. The developed bioreporter system is simple, low-cost, performable at room temperature and free of sample pre-treatment. The findings of this study will facilitate future research for the creation of portable and user-friendly devices for on-site and real-time environmental formaldehyde gas detection.


Inorganic Cadmium Detection Using a Fluorescent Whole-Cell Bacterial Bioreporter
Elcin, Evrim; Öktem, Hüseyin Avni (Informa UK Limited, 2020-11-01)
Cadmium pollution has become a serious environmental issue due to its toxicity and frequent entrance into environment components such as soil, water, and air via many anthropogenic sources. Over the last two decades, whole-cell bacterial bioreporters have been acknowledged as bio-sentinels for the determination of toxic heavy metals. Herein a sensitive and quite specific bacterial bioreporter was developed to cope with the need for the rapid and simple determination of cadmium. The construction and characte...
Development of fluorescent whole-cell bacterial bioreporters for detection of inorganic arsenic and cadmium
Elçin, Evrim; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Environmental heavy metal contamination in many regions of the world is a serious problem of ecological health. Fast and constant monitoring of their levels is significant for both preventing their accumulation and taking an immediate action for removal. As an alternative approach to standard laboratory techniques, various biosensor systems for detection of heavy metals have been proposed. Bacterial biosensors hold great promise for in-field detection of heavy metals. In this study, two different whole cell...
Ambient VOC concentrations in the city of Balikesir and its environment
Yalçın, Giyasettin Efdal; Tuncel, Süleyman Gürdal; Department of Environmental Engineering (2013)
The assessment of volatile organic compounds (VOCs) has become an important field of interest in atmospheric pollution. This study quantifies and characterizes the ambient levels and spatial distribution of VOCs in urban and rural areas of Balıkesir. In addition, for the city drinking water dam excess flux from the traffic estimation was done. For these assessments passive sampling of VOCs performed at approximately 50 locations in and around the city of Balıkesir and analyzed with GC-FID system. Tenax TA w...
Strain-enhanced sensitivity of polymeric sensors templated from cholesteric liquid crystals
Batır, Özge; Bat, Erhan; Büküşoğlu, Emre (Royal Society of Chemistry (RSC), 2020-08-01)
Detection of volatile organic compounds (VOCs) is an important issue due to their harmful impact on human health. In this study, we aimed at enhancing the sensitivity of the anisotropic polymeric films templated from cholesteric liquid crystals (CLCs) in the identification of VOCs at concentrations on the order of 100 ppm. To increase sensitivity, we introduced negative strain to the films in the direction parallel to the helical axis and evaluated its effect on the sensitivity. Specifically, we used LC mix...
Near-IR absorbing BODIPY derivatives as glutathione-activated photosensitizers for selective photodynamic action.
Turan, IS; Cakmak, FP; Kahraman, Deniz Cansen; Atalay, Rengül; Akkaya, EU (2014-12-01)
Enhanced spatiotemporal selectivity in photonic sensitization of dissolved molecular oxygen is an important target for improving the potential and the practical applications of photodynamic therapy. Considering the high intracellular glutathione concentrations within cancer cells, a series of BODIPY-based sensitizers that can generate cytotoxic singlet oxygen only after glutathione-mediated cleavage of the electron-sink module were designed and synthesized. Cell culture studies not only validate our design,...
Citation Formats
E. Elcin, F. Ayaydin, and H. A. Öktem, “Formaldehyde biosensing in air using fluorescent bacterial bioreporter cells,” INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: