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Domates ve domates tohumlarında genetik modifikasyonun tanısı
Date
2007-06-01
Author
Gürakan, Candan
Türkoğlu, Selda
Uçkun, Esra
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https://hdl.handle.net/11511/95744
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Department of Biology, Project and Design
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Screening for genetically modified tomatoes & tomato seeds and identification of CRY1AC and SAM-K specific modifications using gene and construct specific PCR
Uçkun, Esra; Gültekin, Güzin Candan; Department of Biotechnology (2007)
This study was carried out to analyze tomato samples and tomato seeds, purchased from different food markets of Turkey randomly, for the presence of genetic modification by using PCR method as it allows more specific detection. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and also with extraction kits. Screening tests of tomatoes were done by targeting 35S promoter, NOS terminator and NptII kanamycin resistance gene with eight different primer sets. Real time PCR is ...
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Tomato has been genetically modified for providing properties such as insect-resistance or delayed-ripening. Tomato seeds purchased from several bazaars and markets were screened for the presence of genetic modification by targeting NptII kanamycin resistance, Nos terminator, and 35S promoter gene regions which are the most commonly transformed gene regions in transgenic plants, and then ripening-delayed tomato seeds were tried to be identified in this study. F type truncated-PG gene and Sam-k gene were sel...
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C. Gürakan, S. Türkoğlu, and E. Uçkun, “Domates ve domates tohumlarında genetik modifikasyonun tanısı,” 2007. Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95744.