Rhizopus oryzae'nin olası hekzokinaz genlerinin klonlanması ve Saccharomyces cerevisiae'de expresyonu

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2013-10-01

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Hamamcı, Haluk
Yücel, Meral

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https://hdl.handle.net/11511/95814

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Department of Food Engineering, Project and Design

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Screening of the putative hexokinase genes from rhizopus oryzae Alagöz, Eda; Hamamcı, Haluk; Yücel, Ayşe Meral; Department of Biotechnology (2013) Rhizopus oryzae is a filamentous fungus which can ferment sugar to ethanol, and lactic acid. Increasing demand of the world for use of renewable carbon sources has put this and similar organisms into a position where the biotechnology industries are more and more interested with these organisms. R. oryzae can grow on some renewable carbon sources which makes it a good candidate for production of ethanol as well as lactic acid. The major use of lactic acid is in food industry and food- related application, w...
Biochemical and genetic studies on the pyruvate branch point enzymes of rhizopus oryzae Açar, Şeyda; Hamamcı, Haluk; Department of Biotechnology (2004) Rhizopus oryzae is a filamentous fungi which produces lactic acid and ethanol in fermentations. R. oryzae has numerous advantages for use industrial production of L-(+)-lactic acid but the yield of lactic acid produced on the basis of carbon consumed is low. Metabolic flux analysis of R. oryzae has shown that most of the pyruvate produced at the end of the glycolysis is channelled to ethanol, acetyl-CoA and oxaloacetate production. This study aimed to answer some questions addressed on the regulation of pyr...
Deletion mutation of GLNB and GLNK genes in rhodobacter capsulatus to enhance biohydrogen production Pekgöz, Gülşah; Gündüz, Ufuk; Eroğlu, İnci; Department of Biotechnology (2010) Rhodobacter capsulatus is a photosynthetic, purple non-sulfur (PNS) bacterium that produces biohydrogen via photofermentation. Nitrogenase enzyme is responsible for hydrogen production; during fixation of molecular nitrogen into ammonium, hydrogen is produced. Since this process is an energetically expensive process for the cell, hydrogen production is strictly controlled at different levels. When ammonium is present in the environment, hydrogen production completely ceases. The key proteins in the regulati...
Characterisation of the genetically modified cytochrome systems and their application to biohydrogen production in rhodobacter capsulatus Öztürk, Yavuz; Yücel, Ayşe Meral; Department of Biotechnology (2005) Facultative phototrophic bacterium Rhodobacter capsulatus has two c-type electron carrier cytochromes (cyt); the soluble cyt c2 and the membrane-attached cyt cy, that act as electron carriers during respiratory and photosynthetic growth of this species. Previously, a soluble form of cyt cy was constructed by fusing genetically the signal sequence of cyt c2 to the cyt c domain of cyt cy. The obtained novel soluble cyt cy (cyt S-cy) was unable to support photosynthetic growth of R. capsulatus but yielded phot...
A Global approach to the hydrogen production, carnon assimilation and nitrogen metabolism of rhodobacter capsulatus by physiological and microarray analyses Afşar, Nilüfer; Yücel, Ayşe Meral; Department of Biology (2012) One of the most important parameters affecting hydrogen production in photofermentation process is the type of carbon and nitrogen sources. For this reason in this research, the effect of different nitrogen sources (5mM ammonium chloride and 2mM glutamate) and acetate concentrations (40‐80mM) on metabolism of Rhodobacter capsulatus were investigated. First of all, physiological experiments were carried out to compare growth, hydrogen production and substrate utilization behaviors of R. capsulatus on differe...

Citation Formats

H. Hamamcı and M. Yücel, “Rhizopus oryzae’nin olası hekzokinaz genlerinin klonlanması ve Saccharomyces cerevisiae’de expresyonu,” 2013. Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95814.