The effects of antioxidants on some rat tissues and membranes

Download
index.pdf
2004
Görgülü, Güvenç
Show full item record
221
views
186
downloads
High blood glucose levels induce metabolic disorders that initiate a sequence of events including renal, arterial, cardiac and retinal disorders. Diabetes mellitus increases oxidative stress in tissues of animals including humans. The resulting oxidative stress might play role in the development of diabetic complications. In the present study, 36 male Wistar rats (250-300g) were divided into 5 groups as Control (n=6), Diabetic (n=7), Diabetic + Vit C (n=7), Diabetic + a-Lipoic acid (n=6) and Diabetic + Combination of Vit C and a-Lipoic acid (n=10). From the livers of all groups cytoplasmic and microsomal membrane fractions were prepared from liver and antioxidant enzymes namely, superoxide dismutase, glutathione peroxidase, catalase and glutathione S-transferase activities were measured. Microsomal lipid peroxidation, total lipid, total protein, reduced glutathione levels of each group was determined and compared. Microsomal fractions were also analyzed by FT-IR spectroscopy. The total protein levels of diabetic rats were found to be decreased significantly (p<0.05) compared to controls and the a-lipoic acid and vitamin C supplemented groups tend to compensate the decreased levels of total proteins. Decreased catalase activity in diabetic group compared to control was restored by a-lipoic acid, vitamin C treatment and/or combination of both. Increased glutathione peroxidase activity was decreased to control levels by the treatement of both a-lipoic acid and vitamin C. Superoxide dismutase activities of diabetic rats were increased (p<0.05) compared to control group. Whereas glutathione S-transferase activities though showed some fluctuations, the results were not statistically significant. Total glutathione levels decreased in all groups significantly (p<0.0.5) compared to control group but any of the agent failed to compensate the reduced levels of glutathione. As an
Life.
http://etd.lib.metu.edu.tr/upload/2/12604947/index.pdf
https://hdl.handle.net/11511/14229
Graduate School of Natural and Applied Sciences, Thesis

Suggestions

The recovery effect of Vitamin C on structural alterations due to Streptozotocin-Induced diabetes in rat testicular tissues
Küçük Baloğlu, Fatma; Guldag Tas, Damla; Yilmaz, Okkes; Severcan, Feride (2023-03-05)
Type I Diabetes is a multisystem disease that causes alterations in carbohydrate, protein, and fat metabolisms due to hyperglycemia. It has an extensive pathology, especially the mechanism involving oxidative stress is still complex. Type I diabetes is correlated with increased formation of free radicals and decreased levels of antioxidant potential. Vitamin C (Vit C) is a powerful antioxidant that participates in antioxidant defense, protecting lipid membranes and proteins from oxidative damage by donating...
The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study
Bozkurt, Ozlem; Bilgin, Mehmet Dincer; Severcan, Feride (Hindawi Limited, 2007)
Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in me...
The association between metabolic syndrome and its components with systemic lupus erythematosus: a comprehensive systematic review and meta-analysis of observational studies
Hallajzadeh, J.; Khoramdad, M.; Izadi, N.; Karamzad, N.; Almasi-Hashiani, A.; Ayubi, E.; Qorbani, M.; Pakzad, R.; Sullman, M. J. M.; Safiri, S. (2018-05-01)
Objectives: Based upon inflammatory-related factors in chronic systemic lupus erythematosus (SLE), as well as the long-term prescription of corticosteroids, metabolic syndrome (MetS) prevalence is expected to be higher in SLE patients than among those without SLE. The aim of this study was to systematically analyze: (1) the worldwide prevalence of MetS in patients with SLE using different criteria, (2) the risk of MetS in patients with SLE compared with those without SLE, and (3) the risk of MetS component ...
The Effects of Clinical and Home‐based Physiotherapy Programs in Secondary Head and Neck Lymphedema
Ozdemir, Kadirhan; Keser, Ilke; Duzlu, Mehmet; Erpolat, Ozge P.; Saranlı, Uluç; Tutar, Hakan (Wiley, 2020-10-28)
Objectives/Hypothesis: To determine the effects of complex decongestive physiotherapy (CDP) and home programs on external lymphedema, staging of lymphedema, fibrosis, and three-dimensional (3D) surface scanning and volume evaluation in head and neck lymphedema. Study Design: A prospective randomized controlled study. Methods: Twenty-one patients were randomly divided into three groups: CDP (n:7), home program (n:7), and control (n:7). Assessment methods were applied at baseline and 4 weeks later for all gro...
The effects of early postnatal ethanol intoxication on retina ganglion cell morphology and the development of retino-geniculate projections in mice
Dursun, İlknur; Jakubowska Doğru, Ewa; Department of Biology (2011)
Experimental and clinical data have documented the adverse effects of perinatal ethanol intoxication on peripheral organs and the central nervous system. There is little known, however, about potential damaging effects of perinatal ethanol on the developing visual system. The purpose of this study was to examine the effects of neonatal ethanol intoxication on RGC morphology, estimate the total number of neurons in RGC layer and dorsolateral geniculate nucleus (dLGN), and on the eye-specific fiber segregatio...
Citation Formats
G. Görgülü, “The effects of antioxidants on some rat tissues and membranes,” Ph.D. - Doctoral Program, Middle East Technical University, 2004.
METU IIS - Integrated Information Service open@metu.edu.tr