Metastatic behaviour of doxorubicin resistant MCF-7 breast cancer cells after Vimentin silencing

Download

index.pdf

Date

2013

Author

Tezcan, Okan

Metadata

Show full item record

Item Usage Stats

280
views

71
downloads

Chemotherapy is one of the common treatments in cancer therapy. The effectiveness of chemotherapy is limited by several factors one of which is the emergence of multidrug resistance (MDR). MDR is caused by the activity of diverse ATP binding cassette (ABC) transporters that pump drugs out of the cells. There are several drugs which have been used in treatment of cancer. One of them is doxorubicin that intercalates and inhibits DNA replication. However, doxorubicin has been found to cause development of MDR in tumors. It has been reported that there is a correlation between multidrug resistance and invasiveness of cancer cells. Vimentin is a type III intermediate filament protein that is expressed frequently in epithelial carcinomas correlating with invasiveness and also poor prognosis of cancer. There are several studies that have shown the connection between expression level of vimentin and invasiveness. In this study, MCF-7 cell line (MCF-7/S), which is a model cell line for human mammary carcinoma, and doxorubicin resistant MCF-7 cell line (MCF-7/Dox) were used. The resistant cell line was previously obtained by stepwise selection in our laboratory. The main purpose of this study was to investigate changes of metastatic behaviour in MCF-7/Dox cell line, after transient silencing of vimentin gene by siRNA. In conclusion, down-regulation of vimentin gene expression in MCF-7/Dox cell lines was expected to change the characteristics in migration and invasiveness shown by migration and invasion assays.

Subject Keywords

Cancer, Breast, Doxorubicin., Vimentin., Gene silencing., Multidrug resistance.

URI

http://etd.lib.metu.edu.tr/upload/12615650/index.pdf
https://hdl.handle.net/11511/22439

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Vimentin silencing effect on invasive and migration characteristics of doxorubicin resistant MCF-7 cells Tezcan, Okan; Gündüz, Ufuk (2014-04-01) Chemotherapy is one of the well-known treatments in cancer therapy. The effectiveness of chemotherapy is limited by several factors one of which is the emergence of multidrug resistance (MDR). One of the major mechanisms of MDR is the activity of several ATP binding cassette (ABC) transporters that pump drugs out of the cells. Doxorubicin intercalates and inhibits DNA replication; it is a powerful chemotherapeutic agent. However, it causes development of MDR in tumor cells. Vimentin is a type III intermedia...
Targeted co-delivery of doxorubicin and TPGS to breast cancer cells by PLGA coated magnetic nanoparticles Metin, Esra; Gündüz, Ufuk; Mutlu, Pelin; Department of Biotechnology (2017) Although conventional chemotherapy is the most common method for cancer treatment, it has several side effects such as neuropathy, alopecia and cardiotoxicity. Since the drugs are given to body systemically, normal cells also effect as cancer cells. However, in recent years, targeted drug delivery has been developed to overcome these drawbacks.The targeting strategy can be changed depending on carrier types, but magnetic nanoparticles are commonly preferred due to their easy targetable features by using ext...
Reversal of breast cancer resistance protein mediated multidrug resistance in MCF7 breast adenocarcinoma cell line Urfalı, Çağrı; Gündüz, Ufuk; Department of Biology (2011) Resistance to various chemotherapeutic agents is a major problem in success of cancer chemotherapy. One of the primary reasons of development of multidrug resistance (MDR) is the overexpression of ATP binding cassette (ABC) transporter proteins. Breast cancer resistance protein (BCRP) belongs to ABC transporter family and encoded by ABCG2 gene. BCRP is mainly expressed in MDR1 (P-glycoprotein) lacking breast cancer cells. Overexpression of BCRP leads to efflux of chemotherapeutic agents at higher rates, the...
The role of 15-LOX-1 in resistance to chemotherapeutics Kazan, Hasan Hüseyin; Gündüz, Ufuk; Department of Biology (2020) Chemotherapy is one of the best options to treat cancer. However, drug resistance can limit the efficacy of chemotherapeutics. There have been several reasons for the cancer drug resistance including the export of the drug from cells, inactivation of drugs by enzymatic processes, mutations that limit the binding of the drugs to the target proteins, resistance to cell death mechanism by cellular manipulations and reorganization of the cell membrane. 15-Lipoxygenase-1 (15-LOX-1) is a member of the lipoxygenas...
Menadione (Vitamin K3) and Doxorubicin loaded liposomes for enhanced anticancer effect by dual treatment Akkad, Suzan; Keskin, Dilek; Özen, Can; Department of Biotechnology (2022-9-02) Chemotherapy is the first line of cancer treatment, but due to its adverse effects, it is set to be limited. Therefore, liposomes are mainly extensively studied to be employed as nanocarriers as they are more advantageous than traditional therapy. They overcome the obstacle of cellular and tissue uptake and improve drugs’ bioavailability, biodistribution and pharmacokinetics. Doxorubicin, an anthracycline drug, is widely used to treat hematological malignancies and solid tumors, to ameliorate its usage a li...

Citation Formats

O. Tezcan, “Metastatic behaviour of doxorubicin resistant MCF-7 breast cancer cells after Vimentin silencing,” M.S. - Master of Science, Middle East Technical University, 2013.