Proteomics Approach to Differentiate Protein Extraction Methods in Sugar Beet Leaves

Date

2023-06-01

Author

Goktayoglu, Ece
Öztop, Halil Mecit
Özcan Kabasakal, Süreyya

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Interest in alternative plant-based protein sources iscontinuouslygrowing. Sugar beet leaves have the potential to satisfy that demanddue to their high protein content. They are considered as agriculturalwaste and utilizing them as protein sources can bring them back tothe food chain. In this study, isoelectric-point-precipitation, heat-coagulation,ammonium-sulfate precipitation, high-pressure-assisted isoelectric-pointprecipitation, and high-pressure-assisted heat coagulation methodswere used to extract proteins from sugar beet leaves. A mass spectrometry-basedproteomic approach was used for comprehensive protein characterization.The analyses yielded 817 proteins, the most comprehensive proteinprofile on sugar beet leaves to date. Although the total protein contentswere comparable, there was a significant difference between the methodsfor low-abundance proteins. High-pressure-assisted methods showedelevated levels of proteins predominantly located in the chloroplast.Here we showed for the first time that the extraction/precipitationmethods may result in different protein profiles that potentiallyaffect the physical and nutritional properties of functional products.

Subject Keywords

plant-based protein, sugar beet leaves, Beta vulgaris, mass spectrometry, proteomics, RuBisCO protein

URI

https://hdl.handle.net/11511/104627

Collections

Department of Food Engineering, Article