Change of microbial community composition in anaerobic digesters during the degradation of nonylphenol diethoxylate

Date

2018-11-01

Author

Murdoch, Fadime Kara
Murdoch, Robert W.
Gültekin, Güzin Candan
Sanin, Faika Dilek

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Nonylphenol polyethoxylate (NPnEO) surfactants, ubiquitous contaminants in sewage processing facilities, continue to receive attention due to their recalcitrance and diverse toxicity concerns. How the addition of nonyiphenol diethoxylate (NP2EO) and its biodegradation products affect microbial community structure in anaerobic semi-continuous digesters was investigated by molecular techniques. Fluorescence in situ hybridization (FISH) and quantitative PCR (qPCR) were used in parallel to monitor total Bacteria, four classes of Proteobacteria, total Archaea, and two genera of acetoclastic methanogens (Methanosarcina and Methanosaeta). Beta- and Gammaproteobacteria were the dominant phyla and the relative abundances of both groups were roughly doubled following NP2EO addition. While Methanosaeta was dominant over Methanosarcina throughout, NP2EO addition and accumulation of NP nearly doubled the relative abundance of Methanosarcina. While digester performance was not affected, this study revealed that relative abundances of the key players in anaerobic digestion were altered by the addition of NP2EO.

Subject Keywords

Waste Management and Disposal, Microbiology, Biomaterials

URI

https://hdl.handle.net/11511/47787

Collections

Department of Food Engineering, Article