Investigation of a Potential APA Regulated Gene in Cancer

2018-09-08
Erson Bensan, Ayşe Elif
Öyken, Merve
Molbiyokon’185 - 8 September 2018 Izmir Biomedicine andGenome CenterHarun Cingoz , Merve Öyken, elif erson bensanregulated APA and how SNX3 might regulate neoplastic phenotypes.Our future work will aim to better understand the consequences of that may have important consequences in cellular signaling pathways. suggest SNX3 to have a pivotal role in modulating receptor levels and dependencies on SNX3 mediated receptor recycling. Our results We examined known receptor cargo molecules to test for their silenced cell line models to examine the phenotypical consequences. consequences in receptor dynamics in cancers. We developed SNX3 SNX3 proteins levels to be deregulated by APA and that might have phospholipids and has a role in receptor recycling. We hypothesized nexin 3) as an APA regulated mRNA. SNX3 binds to endosomal important, especially in cancers. Earlier, we identified SNX3 (sorting regulated by trans factors, consequences of deregulated APA could be signals on pre-mRNAs. Given that these isoforms may be differentially with different 3’UTR lengths due to selection of one of several poly(A) Alternative polyadenylation (APA) generates transcript isoforms 0,0Harun CingözHarun CingözAlternative polyadenylation (APA) generates transcript isoforms with different 3’UTR lengths due to selection of one of several poly(A) signals on pre-mRNAs. Given that these isoforms may be differentially regulated by trans factors, consequences of deregulated APA could be important, especially in cancers. Earlier, we identified SNX3 (sorting nexin 3) as an APA regulated mRNA. SNX3 binds to endosomal phospholipids and has a role in receptor recycling. We hypothesized SNX3 proteins levels to be deregulated by APA and that might have consequences in receptor dynamics in cancers. We developed SNX3 silenced cell line models to examine the phenotypical consequences. We examined known receptor cargo molecules to test for their dependencies on SNX3 mediated receptor recycling. Our results suggest SNX3 to have a pivotal role in modulating receptor levels and that may have important consequences in cellular signaling pathways. Our future work will aim to better understand the consequences of regulated APA and how SNX3 might regulate neoplastic phenotypesKeywords: APA, Membrane Trafficking, mRNA, 3’UTR,
Citation Formats
A. E. Erson Bensan and M. Öyken, “Investigation of a Potential APA Regulated Gene in Cancer,” 2018, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/75652.