Cloning and expression of glucose oxidase gene from aspergillus niger NRRL-3

Sharif, Fadel Akram


Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes from the cephamycin C producer streptomyces clavuligerus
Tunca, Sedef; Özcengiz, Gülay; Piret, Jacqueline; Department of Biotechnology (2002)
The carbon flow through the lysine branch of aspartate pathway is a rate limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, the ask m(aspartokinase) and asd (aspartate semialdehyde dehydrogenase) genes which encode the enzymes catalyzing the first two steps of aspartate pathway were cloned and characterized as the first time from S. clavuligerus NRRL 3585. This was accomplished by constructing four mini-libraries of S. c...
Cloning and initial protein characterization of an estrogen responsive gene: YPEL2
Güpür, Gizem; Muyan, Mesut; Department of Biology (2014)
17β-estradiol (E2), the main circulating estrogen in the body, is involved in physiological regulation of many tissue and organ functions, including mammary tissue. E2 is also involved in target tissue malignancies. E2 regulates cellular proliferation, differentiation and death in target tissues. The lasting effects of E2 on cells are mediated by estrogen receptor and β that are the products of distinct genes and act as transcription factors. Upon binding to E2, the activated ER regulates the expression of ...
Investigation of gmpls applications in optical systems
Göken, Burcu; Ergül, Rüyal; Department of Electrical and Electronics Engineering (2005)
In this study, possible applications of label switching in large area, fully optical networks are investigated. The objective was to design a label assignment method by using Generalized Multi-Protocol Label Switching (GMPLS) concept to get an efficient optical network operation. In order to fulfill this objective, two new approaches were proposed: a label assignment method and a concatenated label structure. Label assignment method was designed to provide an efficient utilization of resources. Concatenated...
Use of the ambiguity function technique for target detection in phase coded continuous wave radars
Çankaya, Erkan; Sayan, Gönül; Department of Electrical and Electronics Engineering (2005)
The goal of this thesis study is to investigate the Ambiguity Function Technique for target detection in phase-coded continuous wave radar. Also, phase shift keying techniques are examined in detail. Continuous Wave (CW) Radars, which are also known as Low Probability of Intercept (LPI) radars, emit continuous signals in time which are modulated by either frequency modulation or phase modulation techniques. Modulation of the transmitted radar signal is needed to estimate both the range and the radial veloci...
Optimization of doppler processing by using bank of matched filters
Aktop, Onur; Tanık, Yalçın; Department of Electrical and Electronics Engineering (2005)
In radars, matched filters are used in the receiver of the system. Since the target velocity is not known a priori, degradation occurs due to mismatch of the return signal and the matched filter. The performance of the radar can be improved by using a bank of matched filters. The first topic investigated in this work is optimization of the bank of matched filter structure. Two methods are proposed for the design of the parallel filter structure and computations are performed with both methods. The output si...
Citation Formats
F. A. Sharif, “Cloning and expression of glucose oxidase gene from aspergillus niger NRRL-3,” Ph.D. - Doctoral Program, Middle East Technical University, 1991.