Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Heavy metal-induced structural and functional changes in clinical and environmental acinetobacter isolates
Download
index.pdf
Date
2014
Author
Özaktaş, Tuğba
Metadata
Show full item record
Item Usage Stats
91
views
78
downloads
Cite This
Heavy metal pollution is a threat resulting from increased anthropogenic activities. Cadmium (Cd), lead (Pb) and silver (Ag) are among the heavily used metals in different industrial areas. The accumulation of these hazardous substances in nature affects all organisms including human. Bacteria can tolerate these toxic heavy metals up to a degree by their intrinsic resistance mechanisms. Heavy metal resistance factors generally assist the spread of resistance to other toxic substances and antibiotics. Acinetobacter species are widely distributed opportunistic pathogens in nature. For assessing the molecular patterns of resistance as well as tolerance of Acinetobacter to heavy metals, an environmental and a clinical isolates were subjected to sub-lethal concentrations of Cd, Pb, and Ag. Extent of molecular changes was measured with ATR-FTIR spectroscopy by using alive intact bacterial cells. There were remarkable differences in molecular changes which manifest themselves as apparent resistance and tolerance strategies. These different strategies then lead to differences in physiologies between the isolates originating from two very different environments. This study showed that Pb was the most influential heavy metal on the cellular molecules; in turn it was the most tolerated one. Especially in environmental strain, Pb and Ag induced the extracellular polysaccharide (EPS) synthesis. Furthermore, one of the noteworthy results of this study is that Pb, in environmental strain, caused formation of multiple strand polyribonucleotide aggregations. Interestingly, membrane dynamics were shaped by Cd and Pb in environmental isolate. In contrast clinical isolate did not exhibit measurable change in membrane dynamics. This study gave evidence on the adaptation to specific environments, by modulating the physiology of a bacterium arising from operating with different strategies. Measurable molecular changes than are attributable to the epigenetic potentials of bacteria which provides selections for modulation.
Subject Keywords
Acinetobacter.
,
Heavy metals.
,
Pollution.
,
Hazardous wastes.
,
Fourier transform infrared spectroscopy.
URI
http://etd.lib.metu.edu.tr/upload/12618617/index.pdf
https://hdl.handle.net/11511/24533
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Comparative effects of biochar-nanosheets and conventional organic-amendments on health risks abatement of potentially toxic elements via consumption of wheat grown on industrially contaminated-soil
Yousaf, Balal; Liu, Guijian; Abbas, Qumber; Ullah, Habib; Wang, Ruwei; Zia-ur-Rehman, Muhammad; Amina, Amina; Niu, Zhiyuan (2018-02-01)
Potentially toxic elements (PTEs) discharge to the soil environment through increased anthropogenic activities is a global threat. These l'Its can have harmful and chronic-persistent health effects on exposed populations through food consumption grown on contaminated soils. Efforts to investigate the transformation mechanism and accumulation behavior of PTEs in soil-plant system and their adverse health effects have focused extensively in previous studies. However, limited studies address biochar nano sheet...
Sustainability Assessments of Urban Railway Systems: Case Study Evaluations in Turkey
YÜKSEL, MÜGE; Tanyer, Ali Murat; Pekeriçli, Mehmet Koray (Springer, 2019-01-01)
There are ongoing efforts to control global warming via reducing CO2 emissions, which are mostly based on human activities. Through life cycle assessment (LCA) carbon footprint (CFP) calculations provide a clear picture of embodied carbon (EC). In this study, the significance of EC estimation and reduction for railway projects are explored. The main motivation is having applicable sustainability strategies for railway systems in Turkey. Since there is no certification system, range or database, these effort...
Sustainability Assessments of Urban Railway Systems: Case Study Evaluations in Turkey
Yüksel, Müge; Tanyer, Ali Murat; Pekeriçli, Mehmet Koray (2019-09-12)
There are ongoing efforts to control global warming via reducing CO2 emissions, which are mostly based on human activities. Through life cycle assessment (LCA) carbon footprint (CFP) calculations provide a clear picture of embodied carbon (EC). In this study, the significance of EC estimation and reduction for railway projects are explored. The main motivation is having applicable sustainability strategies for railway systems in Turkey. Since there is no certification system, range or database, these effort...
Spatial Modelling of Urban Mobility Induced Air Pollution: A Case Study of Istanbul
Şenay Boyacı, Revnak; Şenyel Kürkçüoğlu, Müzeyyen Anıl; Department of City and Regional Planning (2022-11-22)
Air pollution can be defined as the increase of pollutants in the atmosphere to the levels that are harmful to humans, other living organisms and the environment which occurs during production and consumption activities. The problem of air pollution is increasing all over the world as a result of various factors such as drought, topographic conditions, inversion and climatic characteristics, as well as reasons such as population growth in cities, increasing urbanization and industrialization. Similar to the...
Compositional simulation of CO2 storage in a CO2 flooded carbonate reservoir
Bender, S.; Akın, Serhat (null; 2017-01-01)
CO2 emissions can be reduced by storing it in underground geologic formations such as coal seams, mature or depleted hydrocarbon reservoirs and deep saline aquifers. The combination of carbon dioxide enhanced oil recovery and permanent CO2 storage in mature oil reservoirs has the advantage to project economics. In this research, CO2 storage capacity of a CO2 flooded mature oil reservoir was estimated and the contribution of the CO2 injection to oil recovery was determined by conducting a full-field composit...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
T. Özaktaş, “Heavy metal-induced structural and functional changes in clinical and environmental acinetobacter isolates,” Ph.D. - Doctoral Program, Middle East Technical University, 2014.