Adherence of Candida albicans to glow-discharge modified acrylic denture base polymers

Date

2005-07-01

Author

Yildirim, MS
Hasanreisoglu, U
Hasırcı, Nesrin
Sultan, N

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An important aetiologic factor in the pathogenesis of denture-induced stomatitis, is the presence of numerous yeasts, usually Candida albicans, on the fitting surfaces of dentures. In the present study, effect of glow-discharge plasma, a technique applied to increase surface wettability of acrylic resins, on candidial adherence was evaluated. The durability of glow-discharge modification with saliva coating was also evaluated. Samples including control and experimental groups were prepared by using heat compression mould technique. To create a hidrophobicity gradient, experimental groups were exposed to a radiofrequency glow discharge in an O-2 atmosphere under different discharge powers. To characterize the wetting properties, an expression of surface hidrophobicity, contact angle measurements were performed by the sessile drop method. The organism used was C. albicans (ATTC10321). Acrylic samples were coated with unstimulated whole saliva collected from a healthy man. The fungal suspension was poured on saliva-inoculated samples and incubated at 37 degrees for 2 h. The samples were then fixed with glutaraldehyde and Gram stained. Adhered candidial cells were examined by light microscope. Diffuse Reflectance FTIR (DRIFT) and scanning electron-microscope examinations were also performed to evaluate the surface composition and roughness of the test groups. Glow-discharge plasma was found to be an effective means of increasing surface wettability even with salivary pellicle. Amounts of candida cells adhered were significantly higher in all the plasma treated surfaces than the unmodified control group (P < 0.001). It was concluded that improving the surface wettability of acrylic resins by glow-discharge plasma in O-2 atmosphere increased the adherence of the C. albicans.

Subject Keywords

Poly (methylmethacrylate), Acrylic resin, Wettability, Glow discharge, Candida albicans

URI

https://hdl.handle.net/11511/29987

Journal

JOURNAL OF ORAL REHABILITATION

DOI

https://doi.org/10.1111/j.1365-2842.2005.01454.x

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

M. Yildirim, U. Hasanreisoglu, N. Hasırcı, and N. Sultan, “Adherence of Candida albicans to glow-discharge modified acrylic denture base polymers,” JOURNAL OF ORAL REHABILITATION, pp. 518–525, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29987.