Utilization of pretreated molasses for serine alkaline protease production with recombinant Bacillus species

Calik, G
Pehlivan, N
Kalender, N
Ozdamar, TH
Çalık, Pınar
Recombinant Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis , and Bacillus licheniformis were used for the production of serine alkaline protease (SAP) utilizing chemically and/or physically pretreated molasses. The highest enzyme activity was obtained with r- Bacillus subtilis , with the complex medium involving physically treated molasses having 20 kg m(-3) initial sucrose concentration in small-scale, agitation- and heating rate-controlled bioreactors at t=63 h. Effects of oxygen transfer were investigated in 3.5 dm(3) laboratory bioreactors under two different agitation rates with r-B. subtilis . Increase in the oxygen transfer rate increased the observed activity and caused the cultivation time of maximum activity shift to the earlier stages of the fermentation. At Q/V=0.5 vvm and N=750 min(-1) , SAP activity reached 2250 U cm(-3) at t=36 h.


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Citation Formats
G. Calik, N. Pehlivan, N. Kalender, T. Ozdamar, and P. Çalık, “Utilization of pretreated molasses for serine alkaline protease production with recombinant Bacillus species,” CHEMICAL ENGINEERING COMMUNICATIONS, pp. 630–644, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34927.