Anatomical meniscus construct with zone specific biochemical composition and structural organization

Date

2019-10-01

Author

Bahcecioglu, G.
Bilgen, B.
Hasırcı, Nesrin
Hasırcı, Vasıf Nejat

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A PCL/hydrogel construct that would mimic the structural organization, biochemistry and anatomy of meniscus was engineered. The compressive (380 +/- 40 kPa) and tensile modulus (18.2 +/- 0.9 MPa) of the PCL scaffolds were increased significantly when constructs were printed with a shifted design and circumferential strands mimicking the collagen organization in native tissue (p < 0.05). Presence of circumferentially aligned PCL strands also led to elongation and alignment of the human fibrochondrocytes. Gene expression of the cells in agarose (Ag), gelatin methacrylate (GelMA), and GelMA-Ag hydrogels was significantly higher than that of cells on the PCL scaffolds after a 21-day culture. GelMA exhibited the highest level of collagen type I (COL1A2) mRNA expression, while GelMA-Ag exhibited the highest level of aggrecan (AGO) expression (p < 0.001, compared to PCL). GelMA and GelMA-Ag exhibited a high level of collagen type II (COL2A1) expression (p < 0.05, compared to PCL). Anatomical scaffolds with circumferential PCL strands were impregnated with cell-loaded GelMA in the periphery and GelMA-Ag in the inner region. GelMA and GelMA-Ag hydrogels enhanced the production of COL 1 and COL 2 proteins after a 6-week culture (p < 0.05). COL 1 expression increased gradually towards the outer periphery, while COL 2 expression decreased. We were thus able to engineer an anatomical meniscus with a cartilage-like inner region and fibrocartilage-like outer region.

Subject Keywords

Zone-Specific Biochemical Composition, 3D Printing, PCL/Dual Hydrogel, Human Fibrochondrocytes, Circumferential Fiber Orientation

URI

https://hdl.handle.net/11511/31512

Journal

BIOMATERIALS

DOI

https://doi.org/10.1016/j.biomaterials.2019.119361

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

G. Bahcecioglu, B. Bilgen, N. Hasırcı, and V. N. Hasırcı, “Anatomical meniscus construct with zone specific biochemical composition and structural organization,” BIOMATERIALS, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31512.