Clinoptilolite/PCL-PEG-PCL composite scaffolds for bone tissue engineering applications

2017-03-01
Pazarçeviren, Ahmet Engin
Keskin, Dilek
Tezcaner, Ayşen
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The aim of this study was to prepare and characterize highly porous clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) composite scaffolds. Scaffolds with different clinoptilolite contents (10% and 20%) were fabricated with reproducible solvent-free powder compression/particulate leaching technique. The scaffolds had interconnective porosity in the range of 55-76%. Clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)poly(epsilon-caprolactone) scaffolds showed negligible degradation within eight weeks and displayed less water uptake and higher bioactivity than poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) scaffolds. The presence of clinoptilolite improved the mechanical properties. Highest compressive strength (5.6 MPa) and modulus (114.84 MPa) were reached with scaffold group containing 20% clinoptilolite. In vitro protein adsorption capacity of the scaffolds was also higher for clinoptilolite/poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) scaffolds. These scaffolds had 0.95 mg protein/g scaffold adsorption capacity and also higher osteoinductivity in terms of enhanced ALP, OSP activities and intracellular calcium deposition. Stoichiometric apatite deposition (Ca/P = 1.686) was observed during cellular proliferation analysis with human fetal osteoblasts cells. Thus, it can be suggested that clinoptilolite/poly(epsilon-caprolactone)poly(ethylene glycol)-poly(epsilon-caprolactone) composite scaffolds could be promising carriers for enhancement of bone regeneration in bone tissue engineering applications.
Clinoptilolite, PCL-PEG-PCL, Particulate leaching, Solvent-free, Powder compression, Bone tissue engineering
https://hdl.handle.net/11511/37166
JOURNAL OF BIOMATERIALS APPLICATIONS
Department of Engineering Sciences, Article

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Citation Formats
A. E. Pazarçeviren, D. Keskin, and A. Tezcaner, “Clinoptilolite/PCL-PEG-PCL composite scaffolds for bone tissue engineering applications,” JOURNAL OF BIOMATERIALS APPLICATIONS, pp. 1148–1168, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37166.
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