The characterization and differentiation of higher plants by Fourier transform infrared spectroscopy

Gorgulu, Sevgi Turker
Doğan, Musa
Severcan, Feride
Several techniques have been used to identify and classify plants. We proposed Fourier transform infrared (FT-IR) spectroscopy, together with hierarchical cluster analysis, as a rapid and noninvasive technique to differentiate plants based on their leaf fragments. We applied this technique to three different genera, namely, Ranunculus (Ranunculaceae), Acantholmon (Plumbaginaceae), and Astragalus (Leguminoseae). All of these genera are angiosperms and include a large number of species in Turkey. Ranunculus and Acantholimon have ornamental importance, while Astragalus is an important pharmaceutical genus. The FT-IR spectra revealed dramatic differences, which indicated the variations in lipid metabolism, carbohydrate composition, and protein conformation of the genera. Moreover, cell wall polysaccharides including diverse groups could be identified for each genus. Acantholimon was found to have the highest hydrogen capacity in its polysaccharide and proteins. A higher lignin content. and a lower occurrence of decarboxylation and pectin esterification reactions were appointed for Ranunculus and Astragalus compared to Acantholimon. All these results suggested that FT-IR spectroscopy can be successfully applied to differentiate genera, as demonstrated here with Ranunculus, Astragalus, and Acantholimon. In addition, we used this technique to identify the same species from different geographical regions. In conclusion, the current FT-IR study presents a novel method for rapid and accurate molecular characterization and identification of plants based on the compositional and structural differences in their macromolecules.


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New and rapid techniques for the characterization and identification of bacteria would have an important role in clinical microbiology and in food analysis because of an increasing prevalence of infectious diseases and In this work we carried out two approaches. In the first study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier Transform Infrared (FTIR) Spectroscopic technique. In the second study, we investigated the characterization and i...
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Beneficiation process in the Kirka Borax Concentrator in Turkey generates a tailings effluent containing 3-10% solids that are composed mainly of colloidal particles of clay-rock-type gangue minerals and some unrecoverable borax fines. These colloidal particles form very stable aqueous suspensions in the tailings, hindering solid/liquid separation and clean water recovery. This leads to difficulties in the sustainable and environmentally acceptable operation of the concentrator. Flocculation studies on thes...
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Humic acids (HAs) exist ubiquitously in environments and have a variety of functional groups, which allow them to complex with metal ions and pesticides. Furthermore, these interactions can not only alter the environmental behavior, but also influence the removal and transportation of those pollutants. The study of the interaction between Cu (II), VO (II) and Mn (II) with HAs provides environmental information on the oxidation states of paramagnetic metals and their mechanisms of binding to humic acids. Ele...
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Citation Formats
S. T. Gorgulu, M. Doğan, and F. Severcan, “The characterization and differentiation of higher plants by Fourier transform infrared spectroscopy,” APPLIED SPECTROSCOPY, pp. 300–308, 2007, Accessed: 00, 2020. [Online]. Available: