KCl-induced cortical spreading depression waves more heterogeneously propagate than optogenetically-induced waves in lissencephalic brain: an analysis with optical flow tools

Download

10.1038:s41598-020-69669-6.pdf

Date

2020-07-01

Author

Donmez-Demir, Buket
ERDENER, ŞEFİK EVREN
KARATAŞ KURŞUN, HÜLYA
Kaya, Zeynep
Ulusoy, İlkay
DALKARA, TURGAY

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

80
views

183
downloads

Although cortical spreading depolarizations (CSD) were originally assumed to be homogeneously and concentrically propagating waves, evidence obtained first in gyrencephalic brains and later in lissencephalic brains suggested a rather non-uniform propagation, shaped heterogeneously by factors like cortical region differences, vascular anatomy, wave recurrences and refractory periods. Understanding this heterogeneity is important to better evaluate the experimental models on the mechanistics of CSD and to make appropriate clinical estimations on neurological disorders like migraine, stroke, and traumatic brain injury. This study demonstrates the application of optical flow analysis tools for systematic and objective evaluation of spatiotemporal CSD propagation patterns in anesthetized mice and compares the propagation profile in different CSD induction models. Our findings confirm the asymmetric angular CSD propagation in lissencephalic brains and suggest a strong dependency on induction-method, such that continuous potassium chloride application leads to significantly higher angular propagation variability compared to optogenetically-induced CSDs.

Subject Keywords

Multidisciplinary, SPATIOTEMPORAL DYNAMICS, DEPOLARIZATION, CORTEX

URI

https://hdl.handle.net/11511/47997

Journal

SCIENTIFIC REPORTS

DOI

https://doi.org/10.1038/s41598-020-69669-6

Collections

Department of Electrical and Electronics Engineering, Article

Suggestions

OpenMETU
Core

Temporal changes in the gene expression heterogeneity during brain development and aging Isildak, Ulas; Somel, Mehmet; Thornton, Janet M.; Donertas, Handan Melike (Springer Science and Business Media LLC, 2020-03-01) Cells in largely non-mitotic tissues such as the brain are prone to stochastic (epi-)genetic alterations that may cause increased variability between cells and individuals over time. Although increased interindividual heterogeneity in gene expression was previously reported, whether this process starts during development or if it is restricted to the aging period has not yet been studied. The regulatory dynamics and functional significance of putative aging-related heterogeneity are also unknown. Here we ad...
An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos. Seli, E; Lalioti, MD; Flaherty, SM; Sakkas, D; Terzi Çizmecioğlu, Nihal; Steitz, JA (Proceedings of the National Academy of Sciences, 2005-01-11) Gene expression during oocyte maturation, fertilization, and early embryo development until zygotic gene activation is regulated mainly by translational activation of maternally derived mRNAs. This process requires the presence of a poly(A)-binding protein. However, the cytoplasmic somatic cell poly(A)-binding protein (PABP1) is not expressed until later in embryogenesis. We recently identified an embryonic poly(A)-binding protein (ePAB) in Xenopus. ePAB is the predominant cytoplasmic PABP in Xenopus oocyte...
Altitude adaptation in Tibetans caused by introgression of Denisovan-like DNA Huerta-Sanchez, Emilia; et. al. (Springer Science and Business Media LLC, 2014-08-14) As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to local adaptations. One of the most celebrated examples in humans is the adaptation of Tibetans to the hypoxic environment of the high-altitude Tibetan plateau(1-3). A hypoxia pathway gene, EPAS1, was previously identified as having the...
Actin stabilizing compounds show specific biological effects due to their binding mode Wang, Shuaijun; Crevenna, Alvaro H.; Ugur, Ilke; Marıon, Antoıne; Antes, Iris; Kazmaier, Uli; Hoyer, Maria; Lamb, Don C.; Gegenfurtner, Florian; Kliesmetes, Zane; Ziegenhain, Christoph; Enard, Wolfgang; Vollmar, Angelika; Zahler, Stefan (Springer Science and Business Media LLC, 2019-07-05) Actin binding compounds are widely used tools in cell biology. We compare the biological and biochemical effects of miuraenamide A and jasplakinolide, a structurally related prototypic actin stabilizer. Though both compounds have similar effects on cytoskeletal morphology and proliferation, they affect migration and transcription in a distinctive manner, as shown by a transcriptome approach in endothelial cells. In vitro, miuraenamide A acts as an actin nucleating, F-actin polymerizing and stabilizing compo...
Tensor form factors of nucleon in QCD ERKOL, GÜRAY; Özpineci, Altuğ (2011-10-25) We extract the isovector tensor nucleon form factors, which play an important role in understanding the transverse spin structure of the nucleon when related to the quark helicity-flip generalized parton distributions via their first moments. We employ the light-cone QCD sum rules to leading order in QCD and include distribution amplitudes up to twist 6 in order to calculate the three tensor form factors H(T). E(T) and (H) over bar (T). Our results agree well with those from other approaches in the low and ...

Citation Formats

B. Donmez-Demir, Ş. E. ERDENER, H. KARATAŞ KURŞUN, Z. Kaya, İ. Ulusoy, and T. DALKARA, “KCl-induced cortical spreading depression waves more heterogeneously propagate than optogenetically-induced waves in lissencephalic brain: an analysis with optical flow tools,” SCIENTIFIC REPORTS, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47997.