Quantification of Anandamide and 2-Arachidonylglycerol in Plasma Samples: A Short, Non-toxic HPLC Method and Sample Storage

Date

2010-01-01

Author

Ozdurak, Rabia Hurrem
Şeker, Tamay
Korkusuz, Petek
Korkusuz, Feza

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Objective: The endocannabinoid system plays an important modulatory role in brain physiology, pain sensation, appetite regulation, cardiovascular system, female reproductive system and the immune system. The endogenous ligands of cannabinoid receptors, anandamide (ANA) and 2-Arachidonylglycerol (2-AG) have been identified in various mammalian tissues. However, it difficult to quantify them accurately in blood and in tissues since these endogenous cannabinoids are found in small amount and they are metabolized very quickly. In order to quantify ANA and 2-AG from blood accurately, it was aimed (a) to determine pre-analytical conditions in blood sampling procedure, (b) to improve extraction process and quantification of ANA and 2-AG in plasma samples by HPLC, and (c) to determine storage conditions and period of these metabolites.

Subject Keywords

Anandamide, 2-arachidonylglycerol, Plasma, Sample storage, Nontoxic HPLC method

URI

https://hdl.handle.net/11511/55697

Journal

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

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Citation Formats

R. H. Ozdurak, T. Şeker, P. Korkusuz, and F. Korkusuz, “Quantification of Anandamide and 2-Arachidonylglycerol in Plasma Samples: A Short, Non-toxic HPLC Method and Sample Storage,” TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, pp. 279–284, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55697.