Cloning of Two Subunit Genes of Thermoplasma volcanium

Date

2002-10-20

Author

Kocabıyık, Semra

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https://hdl.handle.net/11511/72166

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Cloning of alpha and beta subunit genes of 20s proteasome from thermoplasma volcanium. Seyit, Gönül; Kocabıyık, Semara; Akkaya, Mahinur; Department of Biotechnology (2002)
Cloning, expression and sequencing of citrate synthase from thermoplasma volcanium Çekiç, Çağlar; Kocabıyık, Semra; Department of Biotechnology (2004) In this study first time, we have cloned and sequenced the citrate synthase gene from a thermoacidophilic archaeon Thermoplasma (Tp.) volcanium (Optimum growth temperature of Tp.volcanium is 60oC and optimum pH is 2.0.). For cloning we have followed a PCR based approach. Amplification of citrate synthase gene from chromosomal DNA of Tp.volcanium yielded a product of 1476 bp containing an open reading frame of 1161 bp comprising the structural gene. After ligation of the PCR amplicon to pDrive vector through...
Cloning of an extracellular thermostable acid protease from thermoplasma volcanium e.coli Özel, Hatice; Kocabıyık, Semra; Department of Biological Sciences (2003)
Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes from the cephamycin C producer streptomyces clavuligerus Tunca, Sedef; Özcengiz, Gülay; Piret, Jacqueline; Department of Biotechnology (2002) The carbon flow through the lysine branch of aspartate pathway is a rate limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, the ask m(aspartokinase) and asd (aspartate semialdehyde dehydrogenase) genes which encode the enzymes catalyzing the first two steps of aspartate pathway were cloned and characterized as the first time from S. clavuligerus NRRL 3585. This was accomplished by constructing four mini-libraries of S. c...

Citation Formats

S. Kocabıyık, “Cloning of Two Subunit Genes of Thermoplasma volcanium,” 2002, Accessed: 00, 2021. [Online]. Available: 0.