Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

Download

fermentation-08-00705-v2.pdf

Date

2022-12-01

Author

Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

59
views

111
downloads

First-generation (1G) bioethanol is one of the most used liquid biofuels in the transport industry. It is generated by using sugar- or starch-based feedstocks, while second-generation (2G) bioethanol is generated by using lignocellulosic feedstocks. Distillers’ dried grains with solubles (DDGS) is a byproduct of first-generation bioethanol production with a current annual production of 22.6 million tons in the USA. DDGS is rich in fiber and valuable nutrients contents, which can be used to produce lignocellulolytic enzymes such as cellulases and hemicellulases for 2G bioethanol production. However, DDGS needs a pretreatment method such as dilute acid, ammonia soaking, or steam hydrolysis to release monosaccharides and short-length oligosaccharides as fermentable sugars for use in microbial media. These fermentable sugars can then induce microbial growth and enzyme production compared to only glucose or xylose in the media. In addition, selection of one or more suitable microbial strains, which work best with the DDGS for enzyme production, is also needed. Media optimization and fermentation process optimization strategies can then be applied to find the optimum conditions for the production of cellulases and hemicellulases needed for 2G bioethanol production. Therefore, in this review, a summary of all such techniques is compiled with a special focus on recent findings obtained in previous pieces of research conducted by the authors and by others in the literature. Furthermore, a comparison of such techniques applied to other feedstocks and process improvement strategies is also provided. Overall, dilute acid pretreatment is proven to be better than other pretreatment methods, and fermentation optimization strategies can enhance enzyme production by considerable folds with a suitable feedstock such as DDGS. Future studies can be further enhanced by the technoeconomic viability of DDGS as the on-site enzyme feedstock for the manufacture of second-generation bioethanol (2G) in first-generation (1G) ethanol plants, thus bridging the two processes for the efficient production of bioethanol using corn or other starch-based lignocellulosic plants

Subject Keywords

DDGS, distillers’ dried grains with solubles, lignocellulolytic enzymes, cellulase, hemicellulase, 1G + 2G

URI

https://hdl.handle.net/11511/101559

Journal

Fermentation

DOI

https://doi.org/10.3390/fermentation8120705

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Synthesis of Tungstophosphoric Acid Incorporated Mesoporous Alumina Catalysts for Methanol Dehydration in DME Synthesis Şener, M İlker; Sezgi, Naime Aslı; Doğu, Timur; Doğu, Güşen; Oktar, Nuray (null; 2016-04-27) Dimethyl ether (DME) is considered as a highly promising environmentally clean diesel fuel alternate, which can be produced from synthesis gas. It has high cetane number (55-60) and clean burning properties [1]. Possibility of using CO2 as the carbon source in DME production makes this process even more attractive as a carbon neutral technology. Production of DME from synthesis gas requires a bifunctional catalyst combination, for methanol synthesis and dehydration functions. As for the methanol synthesis ...
A compact tubular photobioreactor for outdoor hydrogen production from molasses KAYAHAN, Emine; Eroglu, Inci; Koku, Harun (2017-01-26) Hydrogen can be produced sustainably by photofermentation of biomass. For an economically feasible operation, the process should be implemented outdoors using low-cost organic material. In the current study, molasses from a sugar factory was utilized for photofermentative hydrogen production. The experiment was run with Rhodobacter capsulatus YO3 (hup-) in fed-batch mode under outdoor conditions in Ankara between July 12, 2015 and July 24, 2015. The stacked U-tube photobioreactor (9 L) designed for outdoor ...
A cyclic steam injection model for gas production from a hydrate reservoir Ceyhan, N; Parlaktuna, Mahmut (2001-06-01) Thermally efficient production of natural gas from a hydrate reservoir by the use of cyclic steam injection process was modeled.
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01) © 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
Development of anodes for direct oxidation of methane fuel in solid oxide fuel cells Akdeniz, Yelda; Timurkutluk, Bora; Timurkutluk, Cigdem (2016-06-22) In addition to pure hydrogen, solid oxide fuel cells (SOFCs) can utilize hydrocarbons as a fuel. However, conventional Ni-based anodes exhibit an excellent catalytic activity towards the hydrocarbon cracking reaction and thus the carbon deposition occurs in the anode. The deposited carbons quickly deactivate the anode irreversibly by covering the active surface of the anode catalyst. As a result, a significant degradation in the cell performance can be seen. In this study, the anode structure is modified by...

Citation Formats

A. Iram, D. Çekmecelioğlu, and A. Demirci, “Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production,” Fermentation, vol. 8, no. 705, pp. 0–0, 2022, Accessed: 00, 2023. [Online]. Available: https://hdl.handle.net/11511/101559.