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Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Date
2022-10-01
Author
Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali
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© 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimization of fungal inoculum size (1–10%), aeration (0.5-2vvm) and agitation rates (100–500rpm) through response surface methodology (RSM) to obtain highest amounts of cellulases and xylanases. Aspergillus niger (NRRL 330) was grown in dilute acid treated DDGS supplemented with peptone, yeast extract, and ammonium sulfate in benchtop bioreactors to produce these enzymes. The optimal fermentation results were determined to be 6.5% inoculum size, 310 rpm agitation rate and 1.4 vvm aeration, which revealed increases of cellulase activity from 0.6 to 0.82 IU/ml and xylanase activity from 3.99 to 52.76 IU/ml after optimization. The results prove the need for optimization of culture conditions such as aeration, agitation and inoculum size for higher production of hydrolytic enzymes by A. niger using DDGS as the main feedstock.
Subject Keywords
Aspergillus niger
,
Cellulase
,
DDGS
,
Hydrolytic enzymes
,
Lignocellulosic biomass
,
Optimization
,
Xylanase
URI
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85140364778&origin=inward
https://hdl.handle.net/11511/100423
Journal
Biocatalysis and Agricultural Biotechnology
DOI
https://doi.org/10.1016/j.bcab.2022.102514
Collections
Department of Food Engineering, Article
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Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Lignocellulolytic enzymes such as cellulases and xylanases are needed on the industrial scales for low cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focuses on the statistical optimization of fungal inoc...
The effect of Dilution, Aeration, and Agitation on Fungal Cellulase and Xylanase Production by DDGS-based Fermentation Media in Stirred Tank Bioreactors
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01)
Fungal cellulases and xylanases are commonly used for biological pretreatment of lignocellulosic biomass into simple sugars, which can then be converted into many value-added products including bioethanol. However, these enzymes are costly to produce and need a feedstock that should be more economical and high in fiber and other nutrients. Distillers dried grains with solubles (DDGS) is the byproduct of corn ethanol production and can be considered an appropriate feedstock. Among various problems in adaptat...
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Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock
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Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
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A. Iram, D. Çekmecelioğlu, and A. Demirci, “Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors,”
Biocatalysis and Agricultural Biotechnology
, vol. 45, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85140364778&origin=inward.