A Viral Enzymatic Reaction Prioritization Tool for Development of Viral Biosensors

Download

HIBIT22_paper_124.pdf

Date

2022-10

Author

Kıyıkçı , Hüseyin Furkan
Anık , Ülkü
Süzek, Barış Ethem

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

131
views

41
downloads

Viruses are small pathogens that are capable of infecting most eukaryotes. Their effect varies from slight to fatal for the infected organism. Viral infections may lead to significant ecological, social, and financial costs. Despite significant advancements in vaccine and virus research, virus viral diseases continue to be a major public health concern. Biosensors, in general, are compact devices that convert bio-recognition processes into measurable signals in a short amount of time. More specifically, viral biosensors are vehicles to detect viruses and are used for clinical diagnosis of viral infections. In this study, we aim to develop a tool that prioritizes the viral enzyme activities for the selected human viruses. The prioritized enzyme activities and their analytes are then used in the development of viral sensors for the respective viruses while decreasing the time, effort, and money spent in wet-lab experiments conducted for viral biosensor development. The tool collects viral enzyme data from public bioinformatics resources including UniProt Knowledgebase and BRENDA Enzyme database and computes the specificity of the enzymatic reactions at different levels of taxa; virus species, family, and genus. Ideally, the identified enzymatic reactions should be specific to one virus species for effectively using its analyte as a viral biosensor. The tool is successfully validated in the development of a neuraminidase-based colorimetric Influenza A biosensor. Grants from University of Muğla Sıtkı Koçman Scientific Research Projects (BAP) Project No: 21/128/01/1/1 are gratefully acknowledged

URI

https://hibit2022.ims.metu.edu.tr/
https://hdl.handle.net/11511/101946

Conference Name

The International Symposium on Health Informatics and Bioinformatics

Collections

Graduate School of Informatics, Conference / Seminar

Suggestions

OpenMETU
Core

Elucidation of the role of GCN2 gene in response to powdery mildew infection Öztürk, İbrahim Kutay; Akkaya, Mahinur S.; Ersoy, Figen; Department of Biotechnology (2012) Plant immune system is entirely based on the immunities of the individual cells in which systemic signals originate from the infection sites. Powdery mildew disease is one of the agents causing these infection sites, resulting in significant yield losses, if disease develops. Understanding the molecular basis of plant-pathogen interactions is the new trend for fighting against plant pathogens, since classical methods used in selection of resistant plants are becoming less and less efficient nowadays. Thus, ...
Development of a new immobilization procedure for detection of stphylococcal enterotoxin B (SEB) and Candida Albicans Ertürkan, Deniz; Özgen, Canan; Külah, Haluk; Department of Chemical Engineering (2012) Fast and accurate detection of pathogens such as bacteria, their toxins and viruses at low concentrations is very important. The conventional techniques are time consuming where expensive equipment is required with a consumption of excess amount of blood from patients. Recently, immunosensors are used for the detection of pathogens because they are miniature, sensitive, biocompatible and require low power. According to the Centers for Disease Control and Prevention (CDCP), 76 million people become ill due t...
New approaches in microbial pathogen detection Kahyaoğlu, Leyla Nesrin (Woodhead Publishing, 2013-01-01) Viruses are common causes of foodborne outbreaks. Viral diseases have low fatality rates but transmission to humans via food is important due to the high probability of consuming fecally contaminated food or water because of poor food handling. Because of the low infectious doses of some foodborne viruses, there is a need for standardization and the development of new sensitive methods for detecting viruses. The focus is on molecular and non-molecular approaches, and emerging methods for the detection of fo...
Advances in microbial food safety Kahyaoğlu, Leyla Nesrin (2013-07-01) Viruses are common causes of foodborne outbreaks. Viral diseases have low fatality rates but transmission to humans via food is important due to the high probability of consuming fecally contaminated food or water because of poor food handling. Because of the low infectious doses of some foodborne viruses, there is a need for standardization and the development of new sensitive methods for detecting viruses. The focus is on molecular and non- molecular approaches, and emerging methods for the detection of f...
Development of amperometric cholinesterase biosensors based on a conducting polymer for detection of anti-dementia drugs and pesticides Turan, Janset; Toppare, Levent Kamil; Özkan, Necati; Department of Polymer Science and Technology (2016) Cholinesterase enzymes are considered as one of the most important enzymes in living organisms and they are targeted by many inhibitors. Cholinesterase inhibitors are potentially toxic however they also have certain applications as treatment of dementia and Alzheimer’s diseases. These inhibitors can bind the active side of the cholinesterase enzymes forming stable complexes. This inhibitory effect can be used to construct fast and sensitive detection system. Also, conducting polymers are known to be well su...

Citation Formats

H. F. Kıyıkçı, Ü. Anık, and B. E. Süzek, “A Viral Enzymatic Reaction Prioritization Tool for Development of Viral Biosensors,” Erdemli, Mersin, TÜRKİYE, 2022, p. 3124, Accessed: 00, 2023. [Online]. Available: https://hibit2022.ims.metu.edu.tr/.