Microfluidic-based blood immunoassays

Date

2023-05-10

Author

TORUL, HİLAL
Arslan, Zeynep Çağlayan
Tezcan, Tuğba
Kayiş, Elif Çalık
Çalımcı, Merve
Gumustas, Aysen
Yıldırım, Ender
Külah, Haluk
TAMER, UĞUR

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Microfluidics enables the integration of whole protocols performed in a laboratory, including sample loading, reaction, extraction, and measurement steps on a single system, which offers significant advantages thanks to small-scale operation combined with precise fluid control. These include providing efficient transportation mechanisms and immobilization, reduced sample and reagent volumes, fast analysis and response times, lower power requirements, lower cost and disposability, improved portability and sensitivity, and greater integration and automation capability. Immunoassay is a specific bioanalytical method based on the interaction of antigens and antibodies, which is utilized to detect bacteria, viruses, proteins, and small molecules in several areas such as biopharmaceutical analysis, environmental analysis, food safety, and clinical diagnostics. Because of the advantages of both techniques, the combination of immunoassays and microfluidic technology is considered one of the most potential biosensor systems for blood samples. This review presents the current progress and important developments in microfluidic-based blood immunoassays. After providing several basic information about blood analysis, immunoassays, and microfluidics, the review points out in-depth information about microfluidic platforms, detection techniques, and commercial microfluidic blood immunoassay platforms. In conclusion, some thoughts and future perspectives are provided.

Subject Keywords

Blood, Immunoassay, Microfluidics, Optical & electrochemical detection, Point of care

URI

https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85149275760&origin=inward
https://hdl.handle.net/11511/102719

Journal

Journal of Pharmaceutical and Biomedical Analysis

DOI

https://doi.org/10.1016/j.jpba.2023.115313

Collections

Department of Mechanical Engineering, Article

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Citation Formats

H. TORUL et al., “Microfluidic-based blood immunoassays,” Journal of Pharmaceutical and Biomedical Analysis, vol. 228, pp. 0–0, 2023, Accessed: 00, 2023. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85149275760&origin=inward.