Microfluidic-based blood immunoassays

Arslan, Zeynep Çağlayan
Tezcan, Tuğba
Kayiş, Elif Çalık
Çalımcı, Merve
Gumustas, Aysen
Yıldırım, Ender
Külah, Haluk
Microfluidics enables the integration of whole protocols performed in a laboratory, including sample loading, reaction, extraction, and measurement steps on a single system, which offers significant advantages thanks to small-scale operation combined with precise fluid control. These include providing efficient transportation mechanisms and immobilization, reduced sample and reagent volumes, fast analysis and response times, lower power requirements, lower cost and disposability, improved portability and sensitivity, and greater integration and automation capability. Immunoassay is a specific bioanalytical method based on the interaction of antigens and antibodies, which is utilized to detect bacteria, viruses, proteins, and small molecules in several areas such as biopharmaceutical analysis, environmental analysis, food safety, and clinical diagnostics. Because of the advantages of both techniques, the combination of immunoassays and microfluidic technology is considered one of the most potential biosensor systems for blood samples. This review presents the current progress and important developments in microfluidic-based blood immunoassays. After providing several basic information about blood analysis, immunoassays, and microfluidics, the review points out in-depth information about microfluidic platforms, detection techniques, and commercial microfluidic blood immunoassay platforms. In conclusion, some thoughts and future perspectives are provided.
Journal of Pharmaceutical and Biomedical Analysis


Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes
Ozalp, V. Cengiz; Cam, Dilek; Hernandez, Frank J.; Hernandez, Luiza I.; Schafer, Thomas; Öktem, Hüseyin Avni (2016-01-01)
A fast, sensitive and ratiometric biosensor strategy for small molecule detection was developed through nanopore actuation. The new platform engineers together, a highly selective molecular recognition element, aptamers, and a novel signal amplification mechanism, gated nanopores. As a proof of concept, aptamer gated silica nanoparticles have been successfully used as a sensing platform for the detection of ATP concentrations at a wide linear range from 100 mu M up to 2 mM.
Synthesis and Characterization of a Luminol Based Chemiluminescent Trimeric System
Kesimal, Busra; Balci, Burcu; Çakal, Deniz; Önal, Ahmet Muhtar; Cihaner, Atilla (2023-01-01)
A luminol based chemiluminescent trimeric system, namely 2,3-dihydro-5,8-di(thiophen-2-yl)phthalazine-1,4-dione (T2B-Lum), bearing thiophene rings as donor units and 2,3-dihydrophthalazine-1,4-dione as an acceptor unit was synthesized in two steps via donor-acceptor-donor approach using two different methods. It was found that T2B-Lum emits chemiluminescent light when exposed to H2O2 in a basic medium, and the presence of substituents and the type of aromatic ring bearing chemiluminescent active group have ...
An approach to measure trace elements in particles collected on fiber filters using EDXRF
ÖZTÜRK, FATMA; Zararsız, Abdullah; Kırmaz, Rıdvan; Tuncel, Süleyman Gürdal (2011-01-15)
A method developed for analyzes of large number of aerosol samples using Energy Dispersive X-Ray Fluorescence (EDXRF) and its performance were discussed in this manuscript. Atmospheric aerosol samples evaluated in this study were collected on cellulose fiber (Whatman-41) filters, employing a Hi-Vol sampler, at a monitoring station located on the Mediterranean coast of Turkey. between 1993 and 2001. Approximately 1700 samples were collected in this period. Six-hundred of these samples were analyzed by instru...
Microextraction versus exhaustive extraction approaches for simultaneous analysis of compounds in wide range of polarity
Mirnaghi, Fatemeh S.; Gorynski, Krzysztof; Rodriguez-Lafuente, Angel; Boyacı, Ezel; Bojko, Barbara; Pawliszyn, Janusz (2013-11-05)
This article discusses comparison of microextraction versus exhaustive extraction approaches for simultaneous extraction of compounds in wide range of polarity at low and high volumes of sample by comparing solid phase extraction (SPE) and solid phase microextraction (SPME). Here, both systems are discussed theoretically and evaluated based on experimental data. Experimental comparisons were conducted in terms of extraction recovery, sensitivity, and selectivity for the extraction of doping agent compounds ...
A Novel System for Measuring Solids Dispersion In Circulating Fluidized Beds
Külah, Görkem; Bi, H T; Lim, C J; Grace, J R (null; 2008-05-08)
A novel, simple and accurate experimental measurement system with fast response was developed to measure transient local solid tracer concentration distributions to obtain information on solids mixing and dispersion in risers of circulating fluidized beds operating at a wide range of operating conditions. Phosphorescent-coated FCC particles, used as the tracer, were activated by high intensity UV light and injected at the return leg of high-density circulating fluidized bed (HDCFB) unit. The tracer concentr...
Citation Formats
H. TORUL et al., “Microfluidic-based blood immunoassays,” Journal of Pharmaceutical and Biomedical Analysis, vol. 228, pp. 0–0, 2023, Accessed: 00, 2023. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85149275760&origin=inward.