Induction of embryogenic tissue and development of somatic embryos in pinus brutia ten

Yıldırım, Tolga
Conifer species are subjected to major time constraints in tree improvement because of their long regeneration cycle and large sizes. However, integration of developing biotechnologies could significantly reduce this time limitation in tree breeding programs. In this regard, somatic embryogenesis (SE) offers a great potential in commercially important Turkish red pine (Pinus brutia TEN.) for rapid production of larger number of clones as well as capture of greater genetic gains. In this study, seven collections were done to sample precotyledonar zygotic embryos for induction of embryogenic tissue (ET) from 15 clones located in Antalya. Afterwards, abscisic acid, carbohydrates, polyethylene glycol (PEG), and gellan gum were tested to obtain mature somatic embryos in maturation experiments. Analyses of variance showed a significant variation among collection dates (43.1% of total variance) and clones studied (18.8% of total variance) for induction of ETs. Overall initiation frequency of ET in this study was 11.6% with clonal range of 4.7 ا 24.1%. Of those tested maturation treatments, 80æM ABA, sucrose and maltose at 3 and 6%, 3.75% PEG combined with 1% gellan gum were found to be suitable for maturation of somatic embryos in Turkish red pine. Sixty nine somatic embryos were obtained from Clone 22, which was one of tested clones. Induction frequencies could be further improved by using different basal media and/or manipulating media components, such as plant growth regulators. For proper maturation of somatic embryos, embryogenic lines need to be screened to find suitable lines, which are developmentally responsive to ABA treatment.


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Selçuk, Feyza; Yücel, Ayşe Meral; Department of Biology (2004)
Salinity is one of the limiting factors of crop development. Together with causing water loss from plant tissues, salinity also leads to ion toxicity. Under salt stress, increase in Ca+2 concentration in cytosol can decrease the deleterious effects of stress. The binding of Ca+2 to calmodulin initiates a signaling cascade involving the activation of certain transcription factors like STO and STZ. This signal transduction pathway regulates transport of proteins that control net Na+ influx across the plasma m...
Genetic and physiology of cold and drought resistance in Turkish red pine (Pinus brutia, Ten.) populations from southern Turkey
Kandemir, Gaye Eren; Kaya, Zeki; Önde, Sertaç; Department of Biology (2002)
The purpose of the study was to determine the pattern of genetic variation in morphological and physiological traits associated with cold and drought stress using seedling from over-exploited and natural populations of Pinus brutia Ten. (Turkish red pine) from southern Turkey. To determine the pattern of genetic variation existing in over-exploited and natural populations, seedlings from 6 populations each with 40 families were raised in Ankara forest nursery from 1998-2000. Three of the populations (Yaylaa...
Genetic relationships among perennial and annual Cicer species growing in Turkey as revealed by allozymes
Sudupak, MA; Kence, Aykut (Springer Science and Business Media LLC, 2004-05-01)
Allozyme polymorphisms were used to assess genetic variation and relationships among ten Cicer species ( annuals and perennials) growing in Turkey. Using seven enzyme systems, 12 putative scorable loci were detected and surveyed for polymorphism in an accession collection including wild and cultivated forms. Variation was generally low within accessions and species, but common between species. Cluster analysis based on the pairwise genetic distance coefficients (Nei 1978) among accessions and species using ...
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Bryk, Jaroslaw; Somel, Mehmet; Lorenc, Anna; Teschke, Meike (Wiley, 2013-03-01)
Divergence of gene expression is known to contribute to the differentiation and separation of populations and species, although the dynamics of this process in early stages of population divergence remains unclear. We analyzed gene expression differences in three organs (brain, liver, and testis) between two natural populations of Mus musculus domesticus that have been separated for at most 3000years. We used two different microarray platforms to corroborate the results at a large scale and identified hundr...
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Kaya, Zeki; Neale, D. B. (Springer Science and Business Media LLC, 1999-3)
Quantitative trait loci (QTLs) for annual height- and diameter-increment growth were estimated in each of two three-generation loblolly pine pedigrees. Annual height-increment was measured in three consecutive years in the BASE pedigree and in four consecutive years in the QTL pedigree. Annual diameter-increment was measured only in the QTL pedigree for two consecutive years. An interval mapping-approach was used to estimate the number of QTLs, the magnitude of QTL effects, and their position on genetic lin...
Citation Formats
T. Yıldırım, “Induction of embryogenic tissue and development of somatic embryos in pinus brutia ten,” Ph.D. - Doctoral Program, Middle East Technical University, 2005.