Measurement of some essentıal elements ın ınfant formulas ınfant follow-on formulas and fortıfıed baby formulas usıng ICP-OES

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2014
Akkeçi, Emrah
Infancy is a period in which a rapid physical, biological, immunological, and mental growth and development occur. Providing appropriate nutritional resources for the infants are crucial for their healthy growth and development. Insufficient food intake or poor bio-availability may lead to certain deficiencies and dysfunctions. Major and trace elements have important effects and functions on human biology and genetics. As these elements are not produced in the body, they are taken by the foods In the present study, it was aimed to decide, develop and validate the analysis methods for sodium, magnesium, phosphorus, potassium, calcium, iron and zinc in infant formulas, infant follow-on formulas and fortified baby formulas, and determine them using ICP-OES. In the present study, microwave digestion, which is a quite effective method for digestion of food products, was used for sample preparation. The samples were analyzed using 2 different ICP-OES instruments. The accuracy of the method was determined using BCR-063R Skim Milk Powder CRM (IRMM, EU) and GBW 10011Wheat CRM (China) CRM. Analysis results of the CRMs were found in confidence interval of the certified value of the CRMs. Student t-test was applied at 95% confidence levels to check accuracy. It is concluded that there is no significant difference between the experimental values and certified values at 95% confidence level. Detection limits were found to be 15 µg/L, 39 µg/L, 45 µg/L, 24 µg/L, 3 µg/L, 3 µg/L and 3 µg/L for sodium, magnesium, phosphorus, potassium, calcium, iron and zinc respectively for ICP-OES 2100 DV. Detection limits were found to be 45 µg/L, 72 µg/L, 150 µg/L, 75 µg/L, 90 µg/L, 15 µg/L and 18 µg/L for sodium, magnesium, phosphorus, potassium, calcium, iron and zinc respectively for ICP-OES 5300 V. The analyses results of the formulas found in the present study were compared with the boundaries specified by international authorities, national authorities and regulations; consistent results were obtained. The analyses results of the formulas were also evaluated statistically using the Student’s t-test. The results were compared with the reference values specified by the manufacturers and it was revealed that results were not significantly different at 95 % confidence level. In addition, the results of the same sample obtained in two different ICP-OES instruments were compared. There is a significant difference between the experimental and certified values at 95% confidence level. However, null hypothesis at 99% confidence level can be accepted through no significant difference between the certified and experimental values.

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Citation Formats
E. Akkeçi, “Measurement of some essentıal elements ın ınfant formulas ınfant follow-on formulas and fortıfıed baby formulas usıng ICP-OES,” M.S. - Master of Science, Middle East Technical University, 2014.