Engineered natural and synthetic polymer surfaces induce nuclear deformation in osteosarcoma cells

Date

2019-02-01

Author

Antmen Altunsoy, Ezgi
Ermiş Şen, Menekşe
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

170
views

0
downloads

Cell-substrate interactions involve constant probing of microenvironment by cells. One of the responses of cells to environmental cues is to change the conformation of their cytoplasm and nucleus. We hypothesized that surface chemistry and topography could be engineered to make these differences significant enough. When designing the substrates that would accentuate these differences, we prepared surfaces carrying cell adhesive biological cues arranged in specific patterns. Collagen type I and poly(lactic acid-co-glycolic acid) (PLGA) were used to represent substrates with biological cues and those without, and these materials were decorated with four square prism micropillars with different dimensions. The nuclear deformations were analyzed using some descriptors. Nucleus area and solidity were the best descriptors in distinguishing the substrates in terms of biological cues, while nucleus area, solidity, and circularity were more sensitive to the interpillar distances. Another distinguishing factor tested was the duration of contact. Nucleus area was the only descriptor sensitive to nuclear deformation change with time. PLGA was more suitable in nuclear conformation analysis while collagen was better in cell adhesion and proliferation. These deformations lead to changes in the molecular processes and further studies are needed to better understand cell mechanobiology. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 366-376, 2019.

Subject Keywords

Micropattern, Biomimetic, Cell-material interaction, Nucleus, Deformation

URI

https://hdl.handle.net/11511/32062

Journal

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

DOI

https://doi.org/10.1002/jbm.b.34128

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective Ermiş Şen, Menekşe; Antmen Altunsoy, Ezgi; Hasırcı, Vasıf Nejat (2018-09-01) Cell-substrate interactions play a crucial role in the design of better biomaterials and integration of implants with the tissues. Adhesion is the binding process of the cells to the substrate through interactions between the surface molecules of the cell membrane and the substrate. There are several factors that affect cell adhesion including substrate surface chemistry, topography, and stiffness. These factors physically and chemically guide and influence the adhesion strength, spreading, shape and fate o...
Thermal analysis of electroinitiated and radiation induced poly(epoxycyclopentanes) and poly(epoxycyclohexanes) by mass spectrometry Hacaloğlu, Jale; Önal, Ahmet Muhtar (1995-01-01) Thermal behaviour of electroinitiated and radiation induced poly(epoxycyclopentanes), PECP, and poly(epoxycyclohexanes), PECH, have been studied by a direct pyrolysis mass spectrometry technique. The mechanism of thermal degradation of the samples prepared by electroinitiation was found to be a radical depolymerization mechanism yielding mainly monomer. The broader temperature ranges of thermal decomposition in the case of polymer samples prepared by irradiation may be attributed to more complicated degrada...
Characterization and prediction of protein interfaces to infer protein-protein interaction networks Keskin, Ozlem; Tunçbağ, Nurcan; GÜRSOY, Attila (2008-04-01) Complex protein-protein interaction networks govern biological processes in cells. Protein interfaces are the sites where proteins physically interact. Identification and characterization of protein interfaces will lead to understanding how proteins interact with each other and how they are involved in protein-protein interaction networks. What makes a given interface bind to different proteins; how similar/different the interactions in proteins are some key questions to be answered. Enormous amount of prot...
Visualizing the protein-protein interactions network in virtual reality and mixed reality environments Şenderin, Büşra; Sürer, Elif; Tunçbağ, Nurcan; Department of Modeling and Simulation (2021-8) Protein-protein interactions (PPI) define the physical contact of two or more protein structures. When these interactions are combined, the protein-protein interaction network (PPIN) is formed. The interactions between protein structures are distinct interactions—they happen in specific binding locations on proteins, and they have a specific biological function that they take on. With these networks, the processes within a cell or a living organism when healthy or diseased can be studied. In this thesis, a ...
Measurement of multi-jet cross sections in proton-proton collisions at a 7 TeV center-of-mass energy Aad, G.; et. al. (2011-11-01) Inclusive multi-jet production is studied in proton-proton collisions at a center-of-mass energy of 7 TeV, using the ATLAS detector. The data sample corresponds to an integrated luminosity of 2.4 pb(-1). Results on multi-jet cross sections are presented and compared to both leading-order plus parton-shower Monte Carlo predictions and to next-to-leading-order QCD calculations.

Citation Formats

E. Antmen Altunsoy, M. Ermiş Şen, and V. N. Hasırcı, “Engineered natural and synthetic polymer surfaces induce nuclear deformation in osteosarcoma cells,” JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, pp. 366–376, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32062.