Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Ecological Succession of Sulfur-Oxidizing Epsilon- and Gammaproteobacteria During Colonization of a Shallow-Water Gas Vent
Download
10.3389fmicb.2018.02970.pdf
Date
2018-12-06
Author
PATWARDHAN, Sushmita
FOUSTOUKOS, Dionysis
Gioyannelli, Donato
Yücel, Mustafa
VETRİANİ, Costantino
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
131
views
122
downloads
Cite This
In this study, we integrated geochemical measurements, microbial diversity surveys and physiological characterization of laboratory strains to investigate substrate-attached filamentous microbial biofilms at Tor Caldara, a shallow-water gas vent in the Tyrrhenian Sea. At this site, the venting gases are mainly composed of CO2 and H2S and the temperature at the emissions is the same as that of the surrounding water. To investigate the composition of the total and active fraction of the Tor Caldara biofilm communities, we collected established and newly formed filaments and we sequenced the 16S rRNA genes (DNA) and the 16S rRNA transcripts (cDNA). Chemoautotrophic sulfur-oxidizing members of the Gammaproteobacteria (predominantly Thiotrichales) dominate the active fraction of the established microbial filaments, while Epsilonproteobacteria (predominantly Sulfurovum spp.) are more prevalent in the young filaments. This indicates a succession of the two communities, possibly in response to age, sulfide and oxygen concentrations. Growth experiments with representative laboratory strains in sulfide gradient medium revealed that Sulfurovum riftiae (Epsilonproteobacteria) grew closer to the sulfide source than Thiomicrospira sp. (Gammaproteobacteria, Thiotrichales). Overall, our findings show that sulfur-oxidizing Epsilonproteobacteria are the dominant pioneer colonizers of the Tor Caldara biofilm communities and that Gammaproteobacteria become prevalent once the community is established. This succession pattern appears to be driven - among other factors - by the adaptation of Epsilon- and Gammaproteobacteria to different sulfide concentrations.
Subject Keywords
Shallow-water gas vent
,
Tyrrhenian Sea
,
Tor Caldara
,
Microbial biofilms
,
Active microbial communities
,
Epsilonproteobacteria
,
Geothermal
URI
https://hdl.handle.net/11511/32381
Journal
FRONTIERS IN MICROBIOLOGY
DOI
https://doi.org/10.3389/fmicb.2018.02970
Collections
Graduate School of Marine Sciences, Article
Suggestions
OpenMETU
Core
Thermal and geochemical investigation of Seyitomer oil shale
Kök, Mustafa Verşan; Hufnagel, Heinz; Sonel, Nurettin (Elsevier BV, 2001-4)
The results of an experimental study on the thermal and organic geochemical investigation of Seyitomer oil shale sample are presented. Thermogravimetry (TG/DTG) and differential scanning calorimetry (DSC) have been used to determine the thermal behaviour of the oil shale sample. On the other hand, organic carbon content, rock-eval pyrolysis, and gas and liquid chromatography experiments were conducted to determine geochemical properties of the oil shale sample.
HYPERTHERMOPHILIC HYDROGEN PRODUCTION BY GEOGLOBUS ACETIVORANS IN MICROBIAL ELECTROLYSIS CELLS
Kaş, Aykut; Yılmazel Tokel, Yasemin Dilşad; Department of Environmental Engineering (2021-9-10)
Utilization of hyperthermophilic microorganisms was suggested to improve reaction rates and insoluble pollutant degradation and minimize the risk of contamination in bioelectrochemical systems (BESs). So far only a small group of hyperthermophilic microorganisms were identified, which show the ability to donate electrons to solid electrodes in BESs and here we present a new culture that fits to this description. The iron reducing archaeal culture Geoglobus acetivorans, originally isolated from a hydrotherma...
Organic acid production from the organic fraction of municipal solid waste in leaching bed reactors
Doğan, Evrim; Demirer, Göksel Niyazi; Department of Environmental Engineering (2006)
This study was carried out to evaluate the potential of high-rate anaerobic digestion of high-solids organic fraction of municipal solid waste (OFMSW) for the production of organic acids and alcohols in leaching bed reactors (LBRs). For this purpose, two different experimental set-ups, namely Set-1 and Set-2, were operated. In the Set-1, only OFMSW without paper was studied in two identical LBRs, whereas, four identical LBRs, fed with OFMSW with paper and cow manure in different proportions, were operated i...
Differential activation of immune cells by commensal versus pathogen-derived bacterial DNA
Günalp, Sinem; Gürsel, Mayda; Department of Biology (2015)
Immunological mechanisms making contribution to discriminating signals obtained from commensal versus pathogenic bacteria is an active area of research and recent evidence proposes that commensals and pathogens might express discrete variants of pathogen associated molecular patterns (PAMP). We hypothesized that as a major member of PAMP, bacterial DNA (bacDNA) originating from commensals versus pathogens might possess distinct immunostimulatory activities, enabling their dis- crimination by the immune syst...
Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR)
Kocabıyık, Semra (2002-08-01)
In this study 24 thermoacidophilic archeal and bacterial strains isolated from hot-springs and hot-soils were screened for their ability to produce intracellular alkaline proteases. The protease activities of the strains, based on azocasein hydrolysis, showed a variation from 0.6 to 5.1 U. The cell extracts of three most potent producers were further examined and it was found that their proteases exhibited maximum activity at 60-70 degreesC and showed a pH Optimum over a range of pH 7.0-8.5. Gelatin zymogra...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. PATWARDHAN, D. FOUSTOUKOS, D. Gioyannelli, M. Yücel, and C. VETRİANİ, “Ecological Succession of Sulfur-Oxidizing Epsilon- and Gammaproteobacteria During Colonization of a Shallow-Water Gas Vent,”
FRONTIERS IN MICROBIOLOGY
, pp. 0–0, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32381.