Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR)
Date
2002-08-01
Author
Kocabıyık, Semra
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
188
views
0
downloads
Cite This
In this study 24 thermoacidophilic archeal and bacterial strains isolated from hot-springs and hot-soils were screened for their ability to produce intracellular alkaline proteases. The protease activities of the strains, based on azocasein hydrolysis, showed a variation from 0.6 to 5.1 U. The cell extracts of three most potent producers were further examined and it was found that their proteases exhibited maximum activity at 60-70 degreesC and showed a pH Optimum over a range of pH 7.0-8.5. Gelatin zymography revealed that two of the selected archeal strains produced multiple active SDS-resistant proteases. On the other hand, PCR amplification of alkaline serine protease gene sequences of total DNA from all isolates yielded four distinct amplification fragments of 650, 450, 400 and 300 bp, which might have been derived from different serine protease genes.
Subject Keywords
Alkaline serin protease
,
Thermoacidophiles
,
Archaea
URI
https://hdl.handle.net/11511/43046
Journal
BIORESOURCE TECHNOLOGY
DOI
https://doi.org/10.1016/s0960-8524(02)00019-6
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Protease secretion capacity and perforce analysis of recombinant Bacillus species
Çalık, Pınar; Ozdamar, TH (2000-10-08)
Recombinant Bacillus species carrying subC gene encoding serine alkaline protease (SAP) enzyme were developed in order to increase the yield and selectivity in the bioprocess for SAP production. subC gene was amplified from the chromosomal DNA of the wild-type Bacillus licheniformis by using PCR technology; thereafter, subC gene was first cloned into the pRS316 E. coli yeast shuttle plasmid, then sub-cloned into the pHV1431 E. coli-Bacillus shuttle vector, and transferred to the host Bacillus species, i.e. ...
Protoplast isolation and culture in sugar beet (Beta vulgaris L.)
Gürel, Ekrem; Gürel, Songül; Kaya, Zeki (2002-03-01)
In this study, attempts were made to optimise conditions for protoplast isolation from suspension and mesophyll cells, growth of isolated protoplasts and formation of callus from the cultured protoplasts. The enzyme mixture containing 1.0% cellulase, 0.1% hemicellulase, 1.0% pectinase and 0.1% driselase was found to be the most suitable combination for yield and viability of both suspension-and mesophyll-derived protoplasts from sugar beet. Genotypic variation for plating efficiency was significant; ELK345a...
Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49
Bayram, Sinan; Dengiz, Çağatay; GERÇEK, Yusuf Can; ÇETİN, İdil; TOPÇUL, Mehmet Rıfkı (Springer Science and Business Media LLC, 2020-01-01)
In this study, the structure of the purified extracellular eumelanin pigment isolated from Streptomyces spp. was elucidated by detailed analysis via two different spectroscopic techniques (FT-IR and NMR). In vitro antiproliferative effects of eumelanin were evaluated on HeLa cell line. These experiments were carried out with the evaluation of the parameters including cell viability, cell index, and mitotic index. With the cell viability and cell index, IC50 concentration of eumelanin was determined as 10 μM...
Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis
Çalık, Pınar; Özdamar, Tunçer H. (2000-08-01)
The effects of oxygen transfer on the production and product distribution in serine alkaline protease (SAP) fermentation by Bacillus licheniformis and oxygen-transfer strategy in relation to the physiology of the bacilli were investigated on a defined medium with citric acid as sole carbon source in 3.5-dm(3) batch bioreactor systems. By forming a 3 x 3 matrix with the parameters air-inlet rates of Q(O)/V-R = 0.2, 0.5, 1.0 vvm, and agitation rates of N = 150, 500, 750 min(-1), the effects of oxygen transfer...
Thermostability and regulation of Clostridium thermocellum L-lactate dehydrogenase expressed in Escherichia coli
Assa, P; Ozkan, M; Özcengiz, Gülay (2005-01-01)
In this study, L-lactate dehydrogenase (L-LDH) of Clostridium thermocellum previously cloned aid expressed in Escherichia coli FMJ39 was partially purified and characterised. Optimum temperature and pH of the enzyme were found as 50 degrees C and 7.5, respectively. Different concentrations of Mn2+ did not affect the enzyme activity. Addition of 20-30 mM Mg2+, or the other hand, increased the LDH activity by about 10%. Relatively high concentrations of NaCl (2 M), fructose-1,6-diphosphate (FDP, 5 mM), ATP (1...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. Kocabıyık, “Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR),”
BIORESOURCE TECHNOLOGY
, pp. 29–33, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43046.