Monitoring of Tryptophan as a Biomarker for Cancerous Cells in Terahertz (THz) Sensing

Altan, Hakan
Gok, Seher
Ozyurt, Ipek
Severcan, Feride
Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. Changes in the concentration of this amino acid can point to identification of cancerous tissues or even confirm symptoms of depression in patients. Therefore it is extremely important to identify and quantify tryptophan concentrations in human blood as well as in in-vivo diagnostic studies. Here a reflection based terahertz pulsed spectroscopy system was used to study the interaction of THz pulses with cancerous cells to gauge the possibility of using L-tryptophan as a biomarker for THz sensing of diseases. Initial measurements were performed on human colon adenocarcinoma cells and human breast cancer cells cultivated on glass slides. The glass slides utilized in the growth process limited the measurements not only to reflection based geometries but also limited the analysis of the samples in the frequency domain due to the highly absorbing nature of glass in the THz region. The useful bandwidth was limited to frequencies below 0.6THz which prohibited us from investigating the effects of L-tryptophan in these samples. Even with the limited frequency range the measurements show that there are slight differences in the transmission of the THz pulse through different samples.


Concentration-Based Measurement Studies of L-Tryptophan Using Terahertz Time-Domain Spectroscopy (THz-TDS)
Ozer, Zeynep; Gok, Seher; Altan, Hakan; Severcan, Feride (SAGE Publications, 2014-01-01)
L-Tryptophan is an extremely important amino acid for a variety of biological functions in living organisms. In this study we were able to measure changes in the concentration of L-tryptophan when incorporated into pellets with polyethylene as a host. The changes were measured both through the characteristic absorption bands of the C11 and C12 bonds in the low terahertz frequency range and using changes in the refractive index where pellets with higher concentrations of L-tryptophan showed higher refractive...
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Cholinesterase enzymes are considered as one of the most important enzymes in living organisms and they are targeted by many inhibitors. Cholinesterase inhibitors are potentially toxic however they also have certain applications as treatment of dementia and Alzheimer’s diseases. These inhibitors can bind the active side of the cholinesterase enzymes forming stable complexes. This inhibitory effect can be used to construct fast and sensitive detection system. Also, conducting polymers are known to be well su...
Induction of Partial EMT with Nutrient Restriction and Lysosomal Alkalinization in Caco-2 Colorectal Cancer Cells
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Limited availability of nutrients to cancer cells can result in metabolic rewiring, manifesting in the activation of processes such as autophagy for survival. Our study shows for the first time that Caco-2 cells incubated in a nutrient-restriction (NR) medium of low glucose, glutamine and serum for 48h were viable but less proliferative, demonstrated robust autophagy induction and lower sensitivity to 5- Fluorouracil. However, the cargo protein p62 was not degraded efficiently, suggesting a slower autop...
Citation Formats
H. Altan, S. Gok, I. Ozyurt, and F. Severcan, “Monitoring of Tryptophan as a Biomarker for Cancerous Cells in Terahertz (THz) Sensing,” 2016, vol. 9703, Accessed: 00, 2020. [Online]. Available: