Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle

Download
2014-10-01
In this study, extraction of phenolic compounds from nettle by microwave and ultrasound was studied. In both microwave and ultrasound-assisted extractions, effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid to solvent ratio (1:10, 1:20, and 1:30 g/mL) on total phenolic content (TPC) were investigated. Effects of different powers (50 % and 80 %) were also studied for ultrasound-assisted extraction. In microwave-assisted extraction, the optimum TPC of the extracts (24.64 +/- 2.36 mg GAE/g dry material) was obtained in 10 min and at 1:30 solid to solvent ratio. For ultrasound-assisted extraction, the condition that gave the highest TPC (23.86 +/- 1.92 mg GAE/g dry material) was 30 min, 1:30 solid to solvent ratio, and 80 % power. Extracts obtained at the optimum conditions of microwave and ultrasound were compared in terms of TPC, antioxidant activity (AA) and concentration of phenolic acids with conventional extraction and maceration, respectively. Microwave reduced extraction time by 67 %. AA of extracts varied between 2.95 +/- 0.01 and 4.48 +/- 0.03 mg DPPH/g dry material among four methods. Major phenolic compounds were determined as naringenin and chlorogenic acid in nettle.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE

Suggestions

Extraction of phenolic compounds from melissa using microwave and ultrasound
INCE, Alev Emine; Şahin, Serpil; Şümnü, Servet Gülüm (2013-01-01)
In this study, extraction of phenolic compounds from melissa by microwave and ultrasound was studied. In both the microwave and ultrasound extractions, the effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid-to-solvent ratio (1:10, 1:20, and 1:30 g mL(-1)) on total phenolic content (TPC) were investigated. Effects of different powers (50% and 80%) were also studied for the ultrasound extraction. As a solvent, water was used. In microwave extractions, the highest TPC of ext...
Microwave-Assisted Extraction of Phenolic Compounds from Caper
DURMAZ, Ezgi; Şümnü, Servet Gülüm; Şahin, Serpil (2015-09-02)
In this study, it was aimed to extract phenolic compounds from caper by using microwave and to compare the results with conventional extraction. For microwave-assisted extraction, power, extraction time, solid to solvent ratio, and solvent type were selected as independent variables. Dependent variables were total phenolic content (TPC), antioxidant activity, and concentration of phenolic compounds. The increase in solvent amount increased TPC. The highest TPC was obtained by using extraction conditions of ...
Folic acid functionalized PEG coated magnetic nanoparticles for targeting anti-cancer drug delivery: Preparation, characterization and cytotoxicity on Doxorubicin, Zoledronic acid and Paclitaxel resistant MCF-7 breast cancer cell lines
Keskin, Tugba; Yalcin, Serap; Gündüz, Ufuk (2018-01-01)
In this study, MNPs were synthesized, coated with biocompatible polyethylene glycol (PEG) and conjugated with folic acid. Crystal and chemical structures, shape, size and magnetic properties of synthesized nanoparticles were characterized. Agglomeration tendency of naked nanoparticles were prevented by oleic acid addition during the synthesis. All synthesized MNPs have been found to exhibit superparamagnetic behavior at 23 degrees C and 37 degrees C. Cytotoxic effects of MNPs were investigated on Doxorubici...
Usage of microwave and ultrasound in the extraction of essential oils and phenolic compounds
İnce, Alev Emine; Şahin, Serpil; Şümnü, Servet Gülüm; Department of Food Engineering (2011)
The objective of this study is to extract phenolic compounds from nettle and melissa by using microwave and ultrasound and to compare these methods with conventional extraction and maceration, respectively. Extraction of melissa essential oil was also studied. In extraction of phenolics, effects of extraction time (5-20 min for microwave; 5-30 min for ultrasound) and solid to solvent ratio (1:10, 1:20, 1:30 g/ml) on total phenolic content (TPC) were investigated for microwave and ultrasound extractions. Dif...
Comparison of benzaldehyde lyase production capacity in recombinant Escherichia coli and recombinant Bacillus species
Kaya, Hande; Çalık, Pınar; Department of Chemical Engineering (2006)
In this study, the benzaldehyde lyase (BAL, EC 4.1.2.38) production in E. coli BL21 (DE3) pLySs as intracellular and in Bacillus species as extracellular were investigated, and comparison of the production capacity of the enzyme in the developed recombinant microorganisms were compared. For this purpose, firstly, PCR amplified bal gene was cloned into pRSETA vector which is under the control of strong T7 promoter and expressed in E. coli BL21 (DE3) pLysS strain. With developed recombinant E. coli BL21 (DE3)...
Citation Formats
A. E. Ince, S. Şahin, and S. G. Şümnü, “Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle,” JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, pp. 2776–2782, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36089.