Multi-pulsed high hydrostatic pressure treatment for inactivation and injury of Escherichia coli

Date

2011-09-01

Author

Pilavtepe-Celik, Mutlu
BUZRUL, Sencer
Alpas, Hami
LARGETEAU, Alain
DEMAZEAU, Gerard

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

162
views

0
downloads

Escherichia coli cells in peptone water were pressurized at 300 MPa at ambient temperature with no holding time (pulse series) and with a total holding duration of 300 s for single- (300 s x 1 pulse) and multi-pulsed (150 s x 2 pulses, 100 s x 3 pulses, 75 s x 4 pulses, 60 s x 5 pulses, 50 s x 6 pulses and 30 s x 10 pulses) high hydrostatic pressure (HHP) treatments. Multi-pulsed HHP treatment with no holding time indicated that as the pulse number increased the number of inactivated and injured cells also increased. Holding time had significant effect on the inactivation of E. coli. There was low inactivation difference between single- and multi-pulsed HHP treatments with holding time. Escherichia coli cells showed at least 1.6 log(10) more reduction on selective medium than the non-selective medium indicating that more than 95 % of the survivors severely injured for both single- and multi-pulsed treatments with holding time. Although the inactivation difference was low between single- and multi-pulsed HHP treatments, storage at 4 A degrees C revealed that there was less recovery from injury for multi-pulsed HHP treatment.

Subject Keywords

Biotechnology, Agronomy and Crop Science, Food Science, Food Animals

URI

https://hdl.handle.net/11511/37294

Journal

JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY

DOI

https://doi.org/10.1007/s00003-010-0647-2

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Use of Weibull frequency distribution model to describe the inactivation of Alicyclobacillus acidoterrestris by high pressure at different temperatures BUZRUL, SENCER; Alpas, Hami; Bozoglu, F (Elsevier BV, 2005-01-01) The survival curves of Alicyclobacillus acidoterrestris by high hydrostatic pressure were obtained at two pressures (350 and 450 MPa) and three temperature levels (35, 45 and 50 degreesC) in BAM broth. Tailing (upward concavity) was observed in all survival curves. Weibull model was fitted to these data and goodness of fit of this model was investigated. Regression coefficients (R-2), root mean square (RMSE) values and residual plot strongly suggested that Weibull model produced good fit to the data. A bett...
Thermal and chemical inactivation of lactococcal bacteriophages BUZRUL, Sencer; OZTURK, Pinar; Alpas, Hami; AKÇELİK, Mustafa (Elsevier BV, 2007-01-01) Ten lactococcal bacteriophages in M 17 broth were treated by heat (72 degrees C, 15 min and 90 degrees C, 5 min), ethanol and isopropanol at concentrations of 10%, 50%. 75% and 100% (v/v). The heat treatments were not sufficient to inactivate all the bacteriophages studied whereas ethanol, which was also more efficient biocide than isopropanol, at a concentration of 75% could inactivate all the phages in I min. The 50% and 75% of ethanol were more effective than 100%, which was also observed for isopropanol...
Respiratory parameters and sugar catabolism of mushroom (Agaricus bisporus Lange) Varoquaux, P; Gouble, B; Barron, C; Yıldız, Fatih (Elsevier BV, 1999-05-01) The respiration rate (RR) of mushroom (Agaricus bisporus Lange, strain X25) under air follows Arrhenius' law with an apparent activation energy of 43 400 J mol(-1) corresponding to a Q(10) of 2.9 between 10 and 20 degrees C. Oxygen from 20 to 1 kPa did not affect RR at temperatures ranging from 5 to 20 degrees C, confirming previous studies. The initial RR of mushroom (same cultivar, flush, growing conditions) measured 24 h after harvesting can vary from 1 to 2 mmol O-2 kg(-1) h(-1) at 10 degrees C. The app...
Real-time benchtop NMR spectroscopy for the online monitoring of sucrose hydrolysis Soyler, Alper; Bouillaud, Dylan; Farjon, Jonathan; Giraudeau, Patrick; Öztop, Halil Mecit (Elsevier BV, 2020-01-01) The online monitoring of chemical reactions by using benchtop Nuclear Magnetic Resonance (NMR) spectroscopy has become increasingly attractive for the past few years. The use of quantitative online NMR spectroscopy is a promising alternative to traditional analytical methods with its rapid, quantitative and non-invasive nature that makes it applicable to complex and diverse biochemical mixtures like food systems. In this study, sucrose hydrolysis by invertase was chosen as a model reaction for online monito...
KINETIC COMPENSATION RELATIONS FOR ASCORBIC-ACID DEGRADATION AND PECTINESTERASE INACTIVATION DURING ORANGE JUICE PASTEURIZATION ULGEN, N; OZILGEN, M (Wiley, 1991-01-01) The thermal degradation kinetics of ascorbic acid and the inactivation kinetics of pectinesterase were studied experimentally with pH-adjusted orange juice. No simple trends were observed in variation of the kinetic parameters with pH, but both kinetic functions agreed with kinetic compensation relations. An isokinetic temperature was observed for ascorbic acid degradation at pH 2.5, 3.0, and 3.5 implying that the same degradation mechanism was valid at these pH values. A large deviation at pH 4.0 from t...

Citation Formats

M. Pilavtepe-Celik, S. BUZRUL, H. Alpas, A. LARGETEAU, and G. DEMAZEAU, “Multi-pulsed high hydrostatic pressure treatment for inactivation and injury of Escherichia coli,” JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY, pp. 343–348, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37294.