Development of an On-Chip Approach for Oligonucleotide Ligation Mediated SNP Detection

Akca, Oya
Oz, M. Tufan
Öktem, Hüseyin Avni


Development of a pcr-based specific method for the detection of aspergillus fumigatus by random cdna cloning
Söyler, Alper; Ögel, Zümrüt Begüm; Department of Food Engineering (2004)
Aspergillus fumigatus is a foodborne and airborne human pathogen which produces mycotoxins such as gliotoxin, helvolic acid, fumigallin, and fumigaclavin. The most common disease caused by A. fumigatus is aspergillosis, which is often fatal, especially among AIDS, cancer, and organ-transplant patients. In this study, random cDNA cloning was performed from a cDNA library of A. fumigatus (IMI 385708) constructed on ? ZAP Express. Some of these clones were selected according to their insert sizes and were furt...
Development of EpCAM Sensitive Surface Functionalization Strategy for CTC Capture in Microfluidic Channels
Ateş, Hatice Ceren; Şen Doğan, Begüm; Özgür, Ebru; Külah, Haluk (null; 2018-06-19)
Dolaşımdaki tümör hücrelerinin (CTC'ler) izolasyonu ve karakterizasyonu, prognoz ve tedaviye yanıtın erken tespiti açısından önemli klinik öneme sahiptir. Ayrıca, CTC'lerin aşağı akış karakterizasyonu, daha iyi hasta sınıflandırmasına ve tedavi kılavuzluğuna yardımcı olabilir. Bununla birlikte, CTC'ler son derece nadirdir (~ 10 CTC / 1010 periferik kan hücresi) ve oldukça hassastır ve izolasyonları için özel teknoloji gereklidir. Hızla gelişen mikroakışkan teknolojiler, nadir hücre izolasyonunda hızlı, düşü...
Development of nucleic acid coated nanoparticle based lateral flow assay for salmonella detection
Arıcı, Müslüm Kaan; Yücel, Ayşe Meral; Öktem, Hüseyin Avni; Department of Biotechnology (2017)
Advances in nanomaterials have promoted the development of biosensor technologies. User friendly, fast, economic, reliable biosensors can be incorporated into diagnostic methods. Without complex laboratory equipment and qualified person, Point of Care (PoC) tests with biosensors can be carried out. Among a variety of biosensors, nucleic acid based biosensors are promising to have high specificity and sensitivity. Thus, in this study, nucleic acids are employed on modifications of nanomaterials during constr...
Development of a loop mediated isothermal amplification (LAMP) based detection platform for genetically modified organism (GMO) detection
Moğol, Ayça Nazlı; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Genetically modified organisms (GMOs) are being widely used worldwide. Every country has a different legislation regarding the allowed events and GMO levels. This creates the great need for GMO detection. In this study, LAMP assay was used for GMO detection owing to its high sensitivity with the genetically modified organisms; Bt11 maize, GT73 Roundup Ready canola, and transgenic Nicotiana tabacum, targeting the sequences most commonly used in GMO constructions; 35S promoter and Figwort mosaic virus (FMV) s...
Development of nucleic acid coated nanoparticle based lateral flow assay for e.coli detection
Bingöl, Doğa; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Foodborne diseases have been a serious issue for all societies since the beginning of humanity. Thousands of people suffer from foodborne diseases (FBD) every year according to WHO. These diseases are important due to their high frequency and the expenditures that they affect community negatively. Both developing and developed countries can be affected from large outbreaks of FBD. Although most strains of Escherichia coli are beneficial for host microbiota, some serotypes can lead to serious foodborne illne...
Citation Formats
O. Akca, M. T. Oz, and H. A. Öktem, “Development of an On-Chip Approach for Oligonucleotide Ligation Mediated SNP Detection,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: