Development of EpCAM Sensitive Surface Functionalization Strategy for CTC Capture in Microfluidic Channels

Date

2018-06-19

Author

Ateş, Hatice Ceren
Şen Doğan, Begüm
Özgür, Ebru
Külah, Haluk

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Dolaşımdaki tümör hücrelerinin (CTC'ler) izolasyonu ve karakterizasyonu, prognoz ve tedaviye yanıtın erken tespiti açısından önemli klinik öneme sahiptir. Ayrıca, CTC'lerin aşağı akış karakterizasyonu, daha iyi hasta sınıflandırmasına ve tedavi kılavuzluğuna yardımcı olabilir. Bununla birlikte, CTC'ler son derece nadirdir (~ 10 CTC / 1010 periferik kan hücresi) ve oldukça hassastır ve izolasyonları için özel teknoloji gereklidir. Hızla gelişen mikroakışkan teknolojiler, nadir hücre izolasyonunda hızlı, düşük maliyetli ve otomatik numune işleme dahil olmak üzere çeşitli avantajlar ve biyolojik hücrelere benzer fiziksel boyutları nedeniyle daha yüksek hassasiyet ve özgüllük sunar. Bu teknolojilerin çoğu, immünoafinite tabanlı CTC yakalama kullanır, epitel hücre yüzeyi biyobelirtecine karşı anti-EpCAM antikoru, CTC'ye özgü hücre yakalama için yaygın olarak kullanılmaktadır. Bu tür uygulamalarda, uygun bir antikor immobilizasyonu, yüksek verimli hücre yakalama için çok önemli bir rol oynar. Bu çalışmada, verimli, seçici ve EpCAM'a duyarlı CTC yakalama için bir oksit yüzey modifikasyon stratejisi geliştirilmiştir. Hücre yakalama etkinliği, bir CTC modeli olarak MCF-7 göğüs kanseri hücre dizisi kullanılarak belirlenmiş ve ortalama hücre yakalama etkinliği, 8 bağımsız deneyden% 89 olarak bulunmuştur. Seçici CTC yakalama, hücre konsantrasyonu oranı 1: 104 olan arka plan lökositleri içeren tamponda spike edilmiş göğüs kanseri hücreleri (MCF-7) kullanılarak gösterildi ve sırasıyla% 90 ve% 87'lik yakalama verimliliği ve özgüllüğü elde edildi. Hücre yakalamada EpCAM ekspresyonunun önemi, farklı EpCAM ekspresyon seviyelerine sahip beş farklı meme kanseri hücre dizisi, yani MDA-MB-231, MDA-MB-468, SKBR3, MCF-7 ve CAMA-1 ile gösterilmiştir. Sonuçlar, hücrelerin bağlanma verimliliğinin, hücre yüzeyindeki EpCAM ekspresyon seviyesi ile orantılı olduğunu gösterdi.

URI

https://hdl.handle.net/11511/82332

Conference Name

Liquid Biopsy Summit, (17 - 19 Haziran 2018),

Collections

Department of Electrical and Electronics Engineering, Conference / Seminar

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Citation Formats

H. C. Ateş, B. Şen Doğan, E. Özgür, and H. Külah, “Development of EpCAM Sensitive Surface Functionalization Strategy for CTC Capture in Microfluidic Channels,” presented at the Liquid Biopsy Summit, (17 - 19 Haziran 2018), California, Amerika Birleşik Devletleri, 2018, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/82332.