Interaction of a novel platinum drug with bovine serum albumin: FTIR and UV-Vis spectroscopy analysis

Korkmaz, Filiz
Altunöz Erdoğan, Deniz
Ozalp-Yaman, Seniz
Platinum complexes have proven to be very effective in cancer treatment. However, severe side effects of these drugs have lead scientists to pursue new platinum complex derivatives. A novel blue platinum compound, called Platinum-Blue (Pt-Blue), is one of the promising candidate platinum compounds to be used for tumor treatment. In this study, the interaction of Pt-Blue with bovine serum albumin (BSA) has been investigated using UV-Vis and FTIR spectroscopy. One of the findings is that the drug-protein interaction type depends on the drug concentration. Though Pt-Blue is attached to the surface of BSA at high concentrations, it interacts with a hydrophobic region of the protein at low concentrations with a binding constant of 1.93 x 10(5) M-1. Spectroscopic results indicate the hydrophobic docking position to be around Trp 213 in domain II, which is surrounded by a number of Asp and Glu. During this interaction, helices such as helix-10, helix-18, helix-19 and helix-24 change orientation and/or partially unfold to make room for the compound. Binding constants at high and low concentrations of Pt-Blue are determined using UV-Vis spectroscopy, which are found to be comparable to cisplatin. FTIR spectroscopy also reveals that the interaction between Pt-Blue and BSA is noncovalent, which makes the candidate drug favorable because it is available for DNA binding while being carried by albumin.


Nanoparticles Based Drug Delivery Systems to Overcome Multidrug Resistance in Cancer The Role of Membrane Lipids Proteins and Carbohydrates
Özlüer, Özlem; Yalçın Azarkan, Serap; Gündüz, Ufuk (null; 2016-09-28)
Multidrug resistance (MDR) is a major problem in success of cancer chemotherapy on tumor cell growth, limits the prolonged and effective use of chemotherapy. The use of nanomaterial based drug carriers in cancer treatment offers exciting opportunities to enhance delivery of therapeutics to the tumor site (1). This is also known as targeted drug delivery providing differential distribution of drugs to the tumor site while significantly reducing the overall toxicity. Here, we critically discuss the role of th...
Reversal of breast cancer resistance protein mediated multidrug resistance in MCF7 breast adenocarcinoma cell line
Urfalı, Çağrı; Gündüz, Ufuk; Department of Biology (2011)
Resistance to various chemotherapeutic agents is a major problem in success of cancer chemotherapy. One of the primary reasons of development of multidrug resistance (MDR) is the overexpression of ATP binding cassette (ABC) transporter proteins. Breast cancer resistance protein (BCRP) belongs to ABC transporter family and encoded by ABCG2 gene. BCRP is mainly expressed in MDR1 (P-glycoprotein) lacking breast cancer cells. Overexpression of BCRP leads to efflux of chemotherapeutic agents at higher rates, the...
The role of 15-LOX-1 in resistance to chemotherapeutics
Kazan, Hasan Hüseyin; Gündüz, Ufuk; Department of Biology (2020)
Chemotherapy is one of the best options to treat cancer. However, drug resistance can limit the efficacy of chemotherapeutics. There have been several reasons for the cancer drug resistance including the export of the drug from cells, inactivation of drugs by enzymatic processes, mutations that limit the binding of the drugs to the target proteins, resistance to cell death mechanism by cellular manipulations and reorganization of the cell membrane. 15-Lipoxygenase-1 (15-LOX-1) is a member of the lipoxygenas...
The Roles of protein kinase d2 in chemoresistant breast cancer cell lines
Alpsoy, Aktan; Gündüz, Ufuk; Department of Biology (2014)
Even though chemotherapy keeps its position as the most preferred and potent strategy of cancer treatment, resistance of tumor to the anti-neoplastic drug poses an obstacle for chemotherapy success. Multidrug resistance (MDR) is a phenomenon that is defined as the intrinsic or acquired resistance against structurally and functionally unrelated drugs. Acquisition of multidrug resistance can be through several distinct mechanisms such as increased drug efflux by ABC transporters, increased drug detoxification...
Preparation and evaluation of polymer based microcarriers for hydrophobic anti-cancer drugs
Demetçi, Demet; Gündüz, Ufuk; Department of Biotechnology (2007)
Chemotherapy is one of the most important treatments for cancer. However, systemic toxicity, drug resistance and unstable kinetics of the drug in the blood are serious problems of chemotherapy. The use of biodegradable polymers for controlled release of anticancer drugs has gained popularity in recent years. Controlled release of drugs from polymeric carriers has some advantages such as improvement in the efficiency of treatment, reduction in systemic toxicity and prevention of the drug resistance that is d...
Citation Formats
F. Korkmaz, D. Altunöz Erdoğan, and S. Ozalp-Yaman, “Interaction of a novel platinum drug with bovine serum albumin: FTIR and UV-Vis spectroscopy analysis,” NEW JOURNAL OF CHEMISTRY, pp. 5676–5685, 2015, Accessed: 00, 2020. [Online]. Available: