Surface Occupancy Plays a Major Role in Cholesterol's Condensing Effect

Date

2013-08-20

Author

Krause, Martin R.
Türkyılmaz, Serhan
Regen, Steven L.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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The condensing power of cholesterol and 5 alpha-cholestane has been examined in liposomal membranes made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Quantitative nearest-neighbor recognition (NNR) analysis and fluorescence measurements using phase-sensitive probe Laurdan have demonstrated that 5 alpha-cholestane exhibits a substantially weaker condensing effect. This fact, in and of itself, provides compelling evidence that cholesterol's condensing effect is critically dependent on having its steroid nucleus at the membrane surface.

Subject Keywords

Phosphatidylcholines, Bilayers

URI

https://hdl.handle.net/11511/44319

Journal

LANGMUIR

DOI

https://doi.org/10.1021/la402263w

Collections

Department of Chemistry, Article

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Citation Formats

M. R. Krause, S. Türkyılmaz, and S. L. Regen, “Surface Occupancy Plays a Major Role in Cholesterol’s Condensing Effect,” LANGMUIR, pp. 10303–10306, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44319.