Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter

Date

2016-07-01

Author

Gunes, Hande
Çalık, Pınar

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Effects of oxygen transfer on recombinant protein production by Pichia pastoris under glyceraldehyde- 3-phosphate dehydrogenase promoter were investigated. Recombinant glucose isomerase was chosen as the model protein. Two groups of oxygen transfer strategies were applied, one of which was based on constant oxygen transfer rate where aeration rate was Q(O)/V = 3 and 10 vvm, and agitation rate was N = 900 min(-1); while the other one was based on constant dissolved oxygen concentrations, C-DO = 5, 10, 15, 20 and 40 % in the fermentation broth, by using predetermined exponential glucose feeding with mu(o) = 0.15 h(-1). The highest cell concentration was obtained as 44 g L-1 at t = 9 h of the glucose fed-batch phase at C-DO = 20 % operation while the highest volumetric and specific enzyme activities were obtained as 4440 U L-1 and 126 U g(-1) cell, respectively at C-DO = 15 % operation. Investigation of specific enzyme activities revealed that keeping C-DO at 15 % was more advantageous with an expense of relatively higher byproduct formation and lower specific cell growth rate. For this strategy, the highest oxygen transfer coefficient and oxygen uptake rate were K(L)a = 0.045 s(-1) and OUR = 8.91 mmol m(-3) s(-1), respectively.

Subject Keywords

Oxygen transfer, GAP promoter, Pichia pastoris, Fermentation, Bioprocess design, Fed-batch culture

URI

https://hdl.handle.net/11511/44801

Journal

BIOPROCESS AND BIOSYSTEMS ENGINEERING

DOI

https://doi.org/10.1007/s00449-016-1584-y

Collections

Department of Chemical Engineering, Article

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Citation Formats

H. Gunes and P. Çalık, “Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 1061–1072, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44801.