Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter
Date
2016-07-01
Author
Gunes, Hande
Çalık, Pınar
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
233
views
0
downloads
Cite This
Effects of oxygen transfer on recombinant protein production by Pichia pastoris under glyceraldehyde- 3-phosphate dehydrogenase promoter were investigated. Recombinant glucose isomerase was chosen as the model protein. Two groups of oxygen transfer strategies were applied, one of which was based on constant oxygen transfer rate where aeration rate was Q(O)/V = 3 and 10 vvm, and agitation rate was N = 900 min(-1); while the other one was based on constant dissolved oxygen concentrations, C-DO = 5, 10, 15, 20 and 40 % in the fermentation broth, by using predetermined exponential glucose feeding with mu(o) = 0.15 h(-1). The highest cell concentration was obtained as 44 g L-1 at t = 9 h of the glucose fed-batch phase at C-DO = 20 % operation while the highest volumetric and specific enzyme activities were obtained as 4440 U L-1 and 126 U g(-1) cell, respectively at C-DO = 15 % operation. Investigation of specific enzyme activities revealed that keeping C-DO at 15 % was more advantageous with an expense of relatively higher byproduct formation and lower specific cell growth rate. For this strategy, the highest oxygen transfer coefficient and oxygen uptake rate were K(L)a = 0.045 s(-1) and OUR = 8.91 mmol m(-3) s(-1), respectively.
Subject Keywords
Oxygen transfer
,
GAP promoter
,
Pichia pastoris
,
Fermentation
,
Bioprocess design
,
Fed-batch culture
URI
https://hdl.handle.net/11511/44801
Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
DOI
https://doi.org/10.1007/s00449-016-1584-y
Collections
Department of Chemical Engineering, Article
Suggestions
OpenMETU
Core
Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis
Çalık, Pınar; Özdamar, Tunçer H. (2000-08-01)
The effects of oxygen transfer on the production and product distribution in serine alkaline protease (SAP) fermentation by Bacillus licheniformis and oxygen-transfer strategy in relation to the physiology of the bacilli were investigated on a defined medium with citric acid as sole carbon source in 3.5-dm(3) batch bioreactor systems. By forming a 3 x 3 matrix with the parameters air-inlet rates of Q(O)/V-R = 0.2, 0.5, 1.0 vvm, and agitation rates of N = 150, 500, 750 min(-1), the effects of oxygen transfer...
Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol
Celik, Eda; Çalık, Pınar; Oliver, Stephen G. (2009-09-01)
Batch-wise sorbitol addition as a co-substrate at the induction phase of methanol fed-batch fermentation by Pichia pastoris (Mut(+)) was proposed as a beneficial recombinant protein production strategy and the metabolic responses to methanol feeding rate in the presence of sorbitol was systematically investigated. Adding sorbitol batch-wise to the medium provided the following advantages over growth on methanol alone: (a) eliminating the long lag-phase for the cells and reaching 'high cell density productio...
Metabolic engineering with a novel promoter in Pichia pastoris for recombinant human growth hormone production: effects of oxygen transfer conditions
Kalender, Özge; Çalık, Pınar; Özdamar, Tunçer; Department of Chemical Engineering (2018)
The objectives of this thesis are investigation of, i) the effects of oxygen transfer conditions on recombinant protein production in Pichia pastoris strains designed with novel naturally occuring pyruvate kinase (PYK) promoter (PPYK) which is a potential promoter for recombinant protein production under low to moderate oxygen transfer conditions, and ii) influences of engineering with single- and multi- copy genes, in fed-batch fermentation processes. Production of recombinant human growth hormone (rhGH) b...
Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: Use of citric acid as the carbon source
Çalık, Pınar (Elsevier BV, 1998-12-01)
The effects of oxygen transfer on serine alkaline protease (SAP) production by Bacillus licheniformis on a defined medium with C-c = 9.0 kg m(-3) citric acid as sole carbon source were investigated in 3.5 dm(3) batch bioreactor systems. The concentrations of the product (SAP) and by-products, i.e., neutral protease, amylase, amino acids, and organic acids were determined in addition to SAP activities. Ar Q(o)/V = 1 vvm airflow rate, the effect of agitation rate on DO concentration, pH, product, and by-produ...
Oxygen transfer effects on recombinant benzaldehyde lyase production
Çalık, Pınar; Ayhan, Peruze; Demir, Ayhan S. (Elsevier BV, 2004-11)
Oxygen transfer (OT) effects on benzaldehyde lyase (BAL) production by recombinant Escherichia coli carrying pUC18::bal gene were investigated on a defined medium with glucose and (NH4)(2)HPO4 as the sole carbon and nitrogen sources respectively, in 3.0 dm(3) bioreactor systems at six different conditions with the parameters, air inlet rate of Q(O)/V-R = 0.5 vvm, and agitation rates of N = 250, 375, 500, 625, 750 min(-1) and at Q(O)/V-R = 0.7 vvm, N = 750 min(-1). Cell concentration (C-X), BAL volumetric an...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
H. Gunes and P. Çalık, “Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter,”
BIOPROCESS AND BIOSYSTEMS ENGINEERING
, pp. 1061–1072, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44801.