Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock

Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali
Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreatment at 121 degrees C is the most effective way of obtaining simple fermentable sugars (0.382 g/g DDGS). The levels of furfural and HMF was also 5.2 mg/g DDGS) and 1.6 mg/g DDGS, respectively, in the dilute sulfuric acid pretreated DDGS. On the other hand, maximum sugar yield for ammonia pretreatment was 0.129 g/g DDGS and 0.055 g/g DDGS for the steam pretreatment, while no significant amounts of furfural and HMF were observed for these two pretreatment methods.


Production of Cellulase and Xylanase Enzymes Using Distillers Dried Grains with Solubles (DDGS) by Trichoderma reeseiat Shake-Flask Scale and the Validation in the Benchtop Scale Bioreactor
Çekmecelioğlu, Deniz (Springer Science and Business Media LLC, 2020-01-01)
Most of the corn-ethanol plants use dry-mill process and generate large volume of distillers dried grains with solubles (DDGS) as the co-product. The DDGS is a promising feedstock with rich content of cellulose and xylan. The goal of this study was to optimize cellulase and xylanase production byTrichoderma reesei(NRRL 6156) using DDGS as the carbon source at shake-flask and validate the results at benchtop bioreactors. The fermentation medium containing varied amounts of dilute-acid pretreated DDGS, yeast ...
Use of xylan, an agricultural by-product, in wheat gluten based biodegradable films: mechanical, solubility and water vapor transfer rate properties
Kayserilioglu, BS; Bakir, U; Yılmaz, Levent; Akkas, N (Elsevier BV, 2003-05-01)
The possibility of using xylan, as an agricultural by-product, for production of composite films in combinations with wheat gluten was investigated. Different levels of xylan (0-40% w/w) were incorporated into wheat gluten to form biodegradable composite films. Films were prepared at pH 4 and 11, and dried at either uncontrolled or controlled conditions. The mechanical properties, solubilities and water vapour transfer rate (WVTR) of the composite films were studied. Films were obtained with added xylan wit...
Microbial biodiesel production by direct methanolysis of oleaginous biomass
Thliveros, Panagiotis; Kiran, Esra Uckun; Webb, Colin (Elsevier BV, 2014-04-01)
Biodiesel is usually produced by the transesterification of vegetable oils and animal fats with methanol, catalyzed by strong acids or bases. This study introduces a novel biodiesel production method that features direct base-catalyzed methanolysis of the cellular biomass of oleaginous yeast Rhodosporidium toruloides Y4. NaOH was used as catalyst for transesterification reactions and the variables affecting the esterification level including catalyst concentration, reaction temperature, reaction time, solve...
Effects of High Hydrostatic Pressure (HHP) Processing and Temperature on Physicochemical Characterization of Insect Oils Extracted from Acheta domesticus (House Cricket) and Tenebrio molitor (Yellow Mealworm)
Ugur, Ahmet Erdem; Bolat, Berkay; Öztop, Halil Mecit; Alpas, Hami (Springer Science and Business Media LLC, 2020-11-01)
Oil portion of Tenebrio molitor (yellow mealworm) and Acheta domesticus (house cricket) were examined and it was investigated how the physicochemical properties of the oils changed with High Hydrostatic Pressure Assisted Extraction (HHP-E) and conventional solvent extraction (CE) with hexane. The effect of HHP-E at 500 MPa and 30 and 40 degrees C for 15 min on the properties of oils was compared with the CE. Following the extraction of oil, fatty acid composition, peroxide value, crystallization and melting...
Selecting the right blood glucose monitor for the determination of glucose during the enzymatic hydrolysis of corncob pretreated with different methods
Bahcegul, Erinc; Tatli, Emre; Haykir, Nazife Isik; Apaydin, Serpil; Bakir, Ufuk (Elsevier BV, 2011-10-01)
In order to assess their accuracy for the determination of glucose during the enzymatic hydrolysis of pretreated lignocellulosic biomass, four different blood glucose monitors (BGMs), each utilizing a different enzymatic mechanism for the determination of glucose, were utilized in an experimental setup, which compares the efficiency of ionic liquid pretreatment with dilute acid and alkaline pretreatments applied on corncob. Among the tested devices, Optium Xceed was found to be the most accurate device for ...
Citation Formats
A. Iram, D. Çekmecelioğlu, and A. Demirci, “Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers’ dried grains with solubles as a potential fermentation feedstock,” BIORESOURCE TECHNOLOGY, pp. 475–481, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45957.