Phylogenetic differentiation of Aegilops and Triticum species by using ATR-FTIR spectroscopy

Date

2012-11-01

Author

Demir, Pinar
Önde, Sertaç
Ozgen, Murat
AVCI BİRSİN, MELAHAT
SEVERCAN, FERİDE

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

189
views

0
downloads

Triticum and Aegilops species belong to the Poaceae family and show common morphological traits. These morphological common traits made it difficult to identify and name the concerned species. In addition there is great disagreement even in nomenclature of genomic make up. Therefore molecular identification of these species becomes the preferred approach. The study in concern aims to make a phylogenetic differentiation of 8 Aegilops and 4 Triticum species by using Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) Spectroscopy. The cluster analysis results of the whole spectra revealed that the species can be differentiated in terms of their genomic structure. Furthermore, the cluster analysis results of lignin band showed that these species can be differentiated by using ATR-FTIR spectroscopy. These results signify that ATR-FTIR spectroscopy can be used to differentiate different wheat species phylogenetically and the data obtained can be used to improve wheat cultivation.

Subject Keywords

Biotechnology, Applied microbiology

URI

https://hdl.handle.net/11511/46907

DOI

https://doi.org/10.1016/j.jbiotec.2012.07.093

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

Vector construction for transformation of tobacco with a NAC-type transcription factor, TaNAC69-1 Eroğlu, Ayten; BALOĞLU, MEHMET CENGİZ; Yucel, Meral (2016-08-10) NAC type transcription factors are a large family of plant transcription factors related to defense, development, biotic and abiotic responses of plants. To construct vectors for tobacco transformation, traditional and Gateway cloning technology were used. For traditional cloning, firstly TaNAC69-1 gene was isolated from Triticum aestivum L. cv. Yuregir-89. After cDNA synthesis, TaNAC69-1 gene was cloned into pJET1.2 cloning vector. To transfer the gene into dicot expression vector, pORE-E3, both pJET1.2 a...
Proteome-wide analysis of the functional roles of Bacilysin biosynthesis in Bacillus subtilis Taşkın Aras, Aslı; Özcengiz, Gülay; Department of Biology (2010) The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Most of these metabolites are small peptides that have unusual components and chemical bonds and synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. Bacilysin, being produced and excreted by certain strains of Bacillus subtilis, is one of the simplest peptide antibiotics known. It is a dipeptide with an N-terminal L-alanine and an unusual...
Homologous expression of aspartokinase (ask) gene in Streptomyces clavuligerus and its hom-deleted mutant: Effects on cephamycin C production Özcengiz, Gülay; Ünsaldi, Eser; Taşkin, Bilgin; Liras, Paloma; Piret, Jacqueline (Informa UK Limited, 2010-05-01) Abstract In this study, the effect of homologous multiple copies of the ask gene, which encodes aspartokinase catalyzing the first step of the aspartate pathway, on cephamycin C biosynthesis in S. clavuligerus NRRL 3585 and its hom mutant was investigated. The intracellular pool levels of aspartate pathway amino acids accorded well with the Ask activity levels in TB3585 and AK39. When compared with the control strain carrying vector alone without any gene insert, amplification of the ask gene in the w...
SURVIVAL KINETICS OF LACTIC-ACID STARTER CULTURES DURING AND AFTER FREEZE-DRYING BOZOGLU, F; OZILGEN, M; BAKIR, U (Elsevier BV, 1987-09-01) Survival kinetics of lactic acid starter cultures were modeled considering the microorganism and external medium interfacial area as the critical factors determining the resistance of the microorganisms to freeze-drying. Surviving fraction of the microorganisms increased with the increasing biomass concentration during freeze-drying, and this is attributed to the mutual shielding effect of the microorganisms against the severe conditions of the external medium. Survival of the microorganisms over the storag...
Metabolic Flux Analysis for Recombinant Protein Production by Pichia pastoris Using Dual Carbon Sources: Effects of Methanol Feeding Rate Celik, Eda; Çalık, Pınar; Oliver, Stephen G. (Wiley, 2010-02-01) The intracellular metabolic fluxes through the central carbon pathways in the bioprocess for recombinant human erythropoietin (rHuEPO) production by Pichia pastoris (Mut(+)) were calculated. to investigate the metabolic effects of dual carbon sources (methanol/sorbitol) and the methanol feed rate, and to obtain a deeper understanding the regulatory circuitry of P. pastoris, using the established stoichiometry-based model containing 102 metabolites and 141 reaction fluxes. Four fed-batch operations with (MS-...

Citation Formats

P. Demir, S. Önde, M. Ozgen, M. AVCI BİRSİN, and F. SEVERCAN, “Phylogenetic differentiation of Aegilops and Triticum species by using ATR-FTIR spectroscopy,” 2012, vol. 161, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46907.