Phylogenetic differentiation of Aegilops and Triticum species by using ATR-FTIR spectroscopy

Demir, Pinar
Önde, Sertaç
Ozgen, Murat
Triticum and Aegilops species belong to the Poaceae family and show common morphological traits. These morphological common traits made it difficult to identify and name the concerned species. In addition there is great disagreement even in nomenclature of genomic make up. Therefore molecular identification of these species becomes the preferred approach. The study in concern aims to make a phylogenetic differentiation of 8 Aegilops and 4 Triticum species by using Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) Spectroscopy. The cluster analysis results of the whole spectra revealed that the species can be differentiated in terms of their genomic structure. Furthermore, the cluster analysis results of lignin band showed that these species can be differentiated by using ATR-FTIR spectroscopy. These results signify that ATR-FTIR spectroscopy can be used to differentiate different wheat species phylogenetically and the data obtained can be used to improve wheat cultivation.


Vector construction for transformation of tobacco with a NAC-type transcription factor, TaNAC69-1
Eroğlu, Ayten; BALOĞLU, MEHMET CENGİZ; Yucel, Meral (2016-08-10)
NAC type transcription factors are a large family of plant transcription factors related to defense, development, biotic and abiotic responses of plants. To construct vectors for tobacco transformation, traditional and Gateway cloning technology were used. For traditional cloning, firstly TaNAC69-1 gene was isolated from Triticum aestivum L. cv. Yuregir-89. After cDNA synthesis, TaNAC69-1 gene was cloned into pJET1.2 cloning vector. To transfer the gene into dicot expression vector, pORE-E3, both pJET1.2 a...
Proteome-wide analysis of the functional roles of Bacilysin biosynthesis in Bacillus subtilis
Taşkın Aras, Aslı; Özcengiz, Gülay; Department of Biology (2010)
The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Most of these metabolites are small peptides that have unusual components and chemical bonds and synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. Bacilysin, being produced and excreted by certain strains of Bacillus subtilis, is one of the simplest peptide antibiotics known. It is a dipeptide with an N-terminal L-alanine and an unusual...
Homologous expression of aspartokinase (ask) gene in Streptomyces clavuligerus and its hom-deleted mutant: Effects on cephamycin C production
Özcengiz, Gülay; Ünsaldi, Eser; Taşkin, Bilgin; Liras, Paloma; Piret, Jacqueline (Informa UK Limited, 2010-05-01)
Abstract In this study, the effect of homologous multiple copies of the ask gene, which encodes aspartokinase catalyzing the first step of the aspartate pathway, on cephamycin C biosynthesis in S. clavuligerus NRRL 3585 and its hom mutant was investigated. The intracellular pool levels of aspartate pathway amino acids accorded well with the Ask activity levels in TB3585 and AK39. When compared with the control strain carrying vector alone without any gene insert, amplification of the ask gene in the w...
Stress Response Kinetics of Two Nisin Producer Strains of Lactococcus lactis spp. lactis
Simsek, Oemer; BUZRUL, Sencer; Akkoc, Nefise; Alpas, Hami; Akcelik, Mustafa (Springer Science and Business Media LLC, 2009-08-01)
The purpose of this study is to determine the survival and nisin production behaviors of two strains of Lactococcus lactis under different stress conditions that represent the food ecosystem. In this respect, the survival ratios of two nisin producers were determined under different pH, temperature, NaCl, and bile salt concentrations. Then, nisin production levels of the strains were determined at each stress conditions. Both strains had similar growth or inactivation patterns under the same stress conditio...
BOZOGLU, F; OZILGEN, M; BAKIR, U (Elsevier BV, 1987-09-01)
Survival kinetics of lactic acid starter cultures were modeled considering the microorganism and external medium interfacial area as the critical factors determining the resistance of the microorganisms to freeze-drying. Surviving fraction of the microorganisms increased with the increasing biomass concentration during freeze-drying, and this is attributed to the mutual shielding effect of the microorganisms against the severe conditions of the external medium. Survival of the microorganisms over the storag...
Citation Formats
P. Demir, S. Önde, M. Ozgen, M. AVCI BİRSİN, and F. SEVERCAN, “Phylogenetic differentiation of Aegilops and Triticum species by using ATR-FTIR spectroscopy,” 2012, vol. 161, Accessed: 00, 2020. [Online]. Available: