Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Bioreaction network flux analysis for industrial microorganisms: A review
Date
2002-01-01
Author
Çalık, Pınar
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
186
views
0
downloads
Cite This
This review focuses on the analysis of the bioreaction networks of the microorgansims used in fermentation processes, by metabolic flux analysis that is the novel tool of biochemical reaction engineering, required for the quantitative analysis of cell metabolism which is under the control of the cell physiology. Metabolic flux analysis (MFA) is based on calculation of intracellular reaction network rates through various reaction pathways either theoretical or by using elaborate experimental data on uptake, excretion, secretion rates, biosynthetic requirements with metabolic stoichiometry - by solving the mass-balance-based mathematical model developed for the bioreaction network components, either at pseudo-steady state or at steady-state. MFA describes the interactions between the cell and the bioreactor with proper emphasis on the metabolic state and the metabolic process in order to fine-tune the bioreactor performance. This analysis can be used to find the critical branch points and bottlenecks in the overall flux distributions, for modifying the medium composition, for improving the bioreactor operation conditions, moreover for calculating the theoretical metabolic capacities of the microorganism, and for selecting the host microorganism. The methodology of the metabolic flux analysis is provided briefly; thereafter, a comprehensive overview of the state-of-the-art pertaining to cell growth and synthesis of biomolecules in the organisms, i.e. Candida lipolytica, Propionibacterium, Candida utilis, Escherichia coli, Corynebacterium glutamicum, Corynebacterium melassecola, Brevibacterium flavum, Penicillium chrysogenum, Saccharomyces cerevisiae, Streptomyces lividans, Aspergilus niger, Aspergilus oryzae, Bacillus subtilis, Bacillus licheniformis, Ashbya gossypii, Lactococcus lactis, Clostridium acetobutylicum, Torulopsis glabrata, Zymomonas mobilis, Haemophilus influenzae, Streptomyces coelicolor, hybridoma cells, baby hamster kidney cells, Chinese hamster ovary cells, and human liver cells, are given in order to understand the cellular metabolism and the physiology to improve the cellular activities of the cells.
Subject Keywords
General Chemical Engineering
URI
https://hdl.handle.net/11511/48108
Journal
REVIEWS IN CHEMICAL ENGINEERING
DOI
https://doi.org/10.1515/revce.2002.18.6.553
Collections
Department of Chemical Engineering, Article
Suggestions
OpenMETU
Core
Chiral seperations by enzyme enhanced ultrafiltration : fractionation ofracemic benzoin
Ölçeroğlu, Ayşe Hande; Yılmaz, Levent; Department of Chemical Engineering (2006)
In this study, a methodology for separation of chiral molecules, by using enhanced ultrafiltration system was developed. Benzoin was the model chiral molecule studied. In the scope of developing this methodology, some parameters were investigated in the preliminary ultrafiltration experiments in order to set the operation conditions for enhanced ultrafiltration experiments. Due to the slight solubility of benzoin in pure water, 15% (v/v) Polyethylene glycol (PEG 400) and 30 % (v/v) Dimethyl sulfoxide (DMSO)...
Vulcan-Supported Pt Electrocatalysts for PEMFCs Prepared using Supercritical Carbon Dioxide Deposition
Bayrakceken, Ayse; Smirnova, Alevtina; Kitkamthorn, Usanee; Aindow, Mark; Tuerker, Lemi; Eroğlu, İnci; ERKEY, CAN (Informa UK Limited, 2009-01-01)
In this study, supercritical carbon dioxide (scCO(2)) deposition was used to prepare vulcan-supported Pt (Pt/Vulcan) electrocatalysts for proton exchange membrane fuel cells (PEMFCs), and the effects of process variables on the properties of the electrocatalysts were investigated. The two different methods used to reduce the organometallic precursor were thermal reduction in nitrogen at atmospheric pressure and thermal reduction in scCO(2). In the former method, the maximum Pt loading achieved was 9%, and t...
Bioreactor operation parameters as tools for metabolic regulations in fermentation processes: influence of pH conditions
Çalık, Pınar; Calik, G; Ozdamar, TH (Elsevier BV, 2003-02-01)
The influence of controlled- and uncontrolled-pH conditions together with the initial pH on the product and by-product distributions and oxygen transfer characteristics, whereupon the process rate limitations in relation to the intracellular reaction rates were investigated in serine alkaline protease (SAP) fermentation process by recombinant Bacillus licheniformis carrying pHV1431::subC on a defined medium with the sole carbon source glucose in the pH range of 6.80-7.25 in batch bioreactors. Although the s...
Dynamic analysis of changes in diffusion and sorption parameters in the reaction of SO2with activated soda ash
Doǧu, Gülsen; Keskin, Alne; Doǧu, Timur (Wiley, 1988-8)
Variations in pore structure cause significant changes in diffusion resistance and adsorption characteristics during the reaction of SO2 with activated soda. Breakthrough and moment analysis of the SO2‐activated soda ash reaction showed that the effective diffusion coefficient of SO2 in the solid product (mostly Na2SO3) was about three times smaller than its value in the original activated soda. About 30% of the diffusion flux of SO2 in the solid product was found to be due to surface diffusion. The adsorpt...
Bioreaction network flux analysis for human protein producing Bacillus subtilis based on genome-scale model
ÖZDAMAR, HASAN TUNÇER; Senturk, Birguel; YILMAZ, OĞUZ; KOCABAŞ, PINAR; Calik, Guezide; Çalık, Pınar (Elsevier BV, 2010-01-01)
To interpret the effect of human protein gene, e.g., human growth hormone gene hGH, on the intracellular bioreaction network of Bacillus subtilis, the intracellular reaction fluxes were calculated by solving the mass-balance-based genome-scale mathematical model, at pseudo-steady state by using bioreactor data. The bioreaction network consists of 1340 reactions including 990 metabolites. Reaction fluxes in B. subtilis carrying pMK4::pre(subC)::hGH (rBsHGH) and B. subtilis carrying merely pMK4 (rBsP) were ca...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
P. Çalık, “Bioreaction network flux analysis for industrial microorganisms: A review,”
REVIEWS IN CHEMICAL ENGINEERING
, pp. 553–596, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48108.