Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens

2014-10-02
Altuner, Ergin Murat
Ceter, Talip
Alpas, Hami
High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300 MPa at room temperature for 5 min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer saline (PBS) buffer pH 7.5, trichloroacetic acid-acetone and Tris-HCl buffer pH 8.8, was also observed, and the results were compared with the conventional pollen protein extraction procedure. As a result, it is concluded that 200 MPa for 5 min has extracted similar amounts of protein compared with the conventional extraction method which lasted for 24 h, which lasted for 24 h. On the other hand, the application time for 200 MPa for 5 min is extremely shorter when it is compared to the conventional extraction method.
HIGH PRESSURE RESEARCH

Suggestions

Effect of high hydrostatic pressure (HHP) on crystal structure of palm stearin emulsions
Sevdın, Sezen; Yucel, Umut; Alpas, Hami (2017-08-01)
High hydrostatic pressure (HHP) treatment was used to control the crystallization behavior of solid lipid nanoparticles stabilized with sodium caseinate (SC) and soy lecithin Phospholipon 80H (80H). Samples were pressurized at 100 and 500 MPa, at 10, 20 and 40 degrees C for 15 min. The stability of emulsions was analyzed by visual observation for gelation, and the change in the droplet size distribution using light scattering measurements after HHP treatment and storage for 28 days at 4 degrees C. The cryst...
Effects of high hydrostatic pressure (HHP) on cellulose hydrolysis and cellulase activity, and its use for peanut hulls hydrolysis
Öztürk, Eylül; Alpas, Hami; Department of Food Engineering (2019)
High hydrostatic pressure (HHP) technique has potential for food preservation purposes and HHP treatment can inactivate enzymes and/or increase their activity. Avicel, the commercial name of microcrystalline cellulose, is a commonly used substrate to observe the activity of cellulase. Cellulase activity is a crucial parameter for the production of alternative renewable fuels. Celluclast 1.5L, a mixture of enzymes, are used for the hydrolysis of cellulose and hemicellulose containing substances. Peanut hull ...
Effect of High Hydrostatic Pressure Treatment (HHPT) on Quality and Shelf Life of Atlantic Mackerel (Scomber scombrus)
ŞENTÜRK, Tugce; Alpas, Hami (Springer Science and Business Media LLC, 2013-09-01)
The ability of high hydrostatic pressure treatment (HHPT) to extend the shelf life of Atlantic mackerel (Scomber scombrus) was assessed in this study. For that purpose, fillets were subjected to pressure treatments at 200, 300, 400 MPa at 5, 10, 15 A degrees C for 5 and 15 min. The influence of pressure treatments on the levels of trimethylamine nitrogen (TMA-N) and thiobarbituric acid (TBA) as well as color changes was investigated. The suitable combinations were determined as 200 MPa, 15 A degrees C for 5...
Effects of pH and feeding strategy on metabolite profiling of beta-lactamase producing bacillus licheniformis
İler, Nazar; Çalık, Pınar; Department of Chemical Engineering (2005)
In this study, the effects of pH and different feeding modes on beta-lactamase production and cell metabolism were investigated with Bacillus licheniformis (ATCC 25972). For this purpose, first, the effects of pH on beta-lactamase activity, cell formation, substrate consumption, as well as intracellular sodium, potassium, ammonium ion, amino acid and organic acid concentrations were investigated in V= 3.0 dm3 batch bioreactors consisting of temperature, pH, foam, stirring rate and dissolved oxygen controls....
Effect of concentrated flaxseed protein on the stability and rheological properties of soybean oil-in-water emulsions
Wang, Bo; Li, Dong; Wang, Li-Jun; Özkan, Necati (2010-02-01)
Flaxseed protein concentrate containing-mucilage (FPCCM) was used to stabilize soybean oil-in-water emulsions. The effects of FPCCM concentration (0.5, 1.0, 1.5% w/v) and oil-phase volume fraction (5, 10, 20% v/v) on emulsion stability and theological properties of the soybean oil-in-water emulsions were investigated. Z-average diameter, zeta-potential, creaming index and theological properties of emulsions were determined. The result showed that FPCCM concentration significantly affected zeta-potential, cr...
Citation Formats
E. M. Altuner, T. Ceter, and H. Alpas, “Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens,” HIGH PRESSURE RESEARCH, pp. 470–481, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48162.