Show/Hide Menu
Hide/Show Apps
anonymousUser
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Videos
Videos
Thesis submission
Thesis submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Contact us
Contact us
Serine alkaline protease overproduction capacity of Bacillus licheniformis
Date
2000-01-01
Author
Çalık, Pınar
Takac, S
Ozdamar, TH
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
6
views
0
downloads
Cite This
A comprehensive metabolic network that considers 147 reaction fluxes and 105 metabolites is used in a mass-Aux-balance-based stoichiometric model for Bacillus licheniformis for serine alkaline protease (SAP) overproduction. The theoretical capacity analysis leading to optimized SAP overproduction was carried out by using a linear constrained optimization technique for several specific growth rates and the variation of the fluxes were calculated by fixing the sole carbon source citrate's uptake rate at 10 mmol/gDW/h. The theoretical data-based capacity analysis was conducted by using the model in combination with the off-line extracellular analyses of the dry cell and the metabolites that were citrate, organic acids, amino acids, and SAP; and the variations in the intracellular fluxes were obtained for the three periods of the batch bioprocess. The flux distribution maps of the analyses showed that the tricarboxylic acid cycle was active and the cells utilized the gluconeogenesis pathway, the pentose phosphate pathway, and the anaplerotic reactions; nevertheless, the glyoxylate shunt and the glycolysis pathway were inactive. The theoretical capacity analysis showed that SAP synthesis flux increased with the decrease in the specific growth rate, and was the highest at mu = 0 h(-1) as 0.0260 mmol/gDW/h. Both in the theoretical capacity and the theoretical data-based capacity analyses, among the fluxes towards the amino acid groups, aspartic acid group had the highest value and aromatic acid group had the lowest flux value; the flux distributions are similar. The Aux values towards SAP was maximum in Period II, whereas it was minimum in Period I. In Period II of the theoretical data-based capacity analysis, the fluxes of alanine and valine are higher than the other amino acid fluxes; and the pyruvate branch point seems to be the potential metabolic engineering site. The results reveal that SAP production can theoretically be increased 1.09, 16.68, and 7.21 folds, respectively, in Periods I, II, and III. The diversions in the pathways and certain metabolic reactions depending on the bioprocess periods and potential strategies for improving SAP production are also discussed. (C) 2000 Elsevier Science Inc. All rights reserved.
Subject Keywords
Serine alkaline protease
,
Metabolic flux analysis
,
B. licheniformis
,
Theoretical capacity
,
Overproduction
URI
https://hdl.handle.net/11511/50603
Journal
ENZYME AND MICROBIAL TECHNOLOGY
DOI
https://doi.org/10.1016/s0141-0229(99)00126-x
Collections
Department of Chemical Engineering, Article
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
P. Çalık, S. Takac, and T. Ozdamar, “Serine alkaline protease overproduction capacity of Bacillus licheniformis,”
ENZYME AND MICROBIAL TECHNOLOGY
, vol. 26, no. 1, pp. 45–60, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/50603.