Biotech in Turkey

Date

2009-10-27

Author

GEÇKİL, HİKMET
Çalık, Pınar

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Subject Keywords

Molecular Medicine, Applied Microbiology and Biotechnology, General Medicine

URI

https://hdl.handle.net/11511/51134

Journal

Biotechnology Journal

DOI

https://doi.org/10.1002/biot.200900155

Collections

Department of Chemical Engineering, Article

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Microarray studies in Bacillus subtilis Kocabaş, Pinar; Çalık, Pınar; Çalik, Güzide; Özdamar, Tunçer H. (Wiley, 2009-10-27) This review focuses on the construction of a global, comprehensive understanding of Bacillus subtilis through microarray studies. The microarray studies in B. subtilis were analysed based on the theme of the work, by mentioning the growth media, bioreactor operation conditions, RNA isolation method, number of data points analysed in exponential or stationary phases, compared genotypes, induction and repression ratios, investigated gene(s) and their positive and/or negative influences. Based on the theme and...
A MEMS-based spiral channel dielectrophoretic chromatography system for cytometry applications Yilmaz, Gurkan; Ciftlik, Ata Tuna; Külah, Haluk (Wiley, 2011-02-01) In this paper design, fabrication, and evaluation of an easy-to-use and low cost dielectrophoretic quantizer are introduced. The device works with standard tools in a biomedical laboratory: a stereo microscope with CCD camera and a voltage supply. A novel spiral microchannel geometry together with the coaxial electrode configuration is established. The device works with a droplet of sample, eliminating microfluidic connections, and external syringes. The proposed geometry decreases the footprint, therefore ...
Regulatory effects of oxygen transfer on overexpression of recombinant benzaldehyde lyase production by Escherichia coli BL21 (DE3) Kaya-Çeliker, Hande; Angardi, Vahideh; Çalık, Pınar (Wiley, 2009-10-27) Effects of oxygen transfer (OT) on benzaldehyde lyase (BAL) production by recombinant E. coli BL21 (DE3) pLySs were investigated at an air‐inlet rate of QO/VR = 0.5 vvm and agitation rates of N = 250, 500, 625, and 750/min; and at constant dissolved oxygen (DO) concentrations of CDO = 0.04 and 0.08 mol/m3, on a glucose based defined medium. The highest cell concentration (CX = 3.0 kg/m3) and volumetric BAL activity (A = 1095 U/cm3) were obtained at CDO = 0.08 mol/m3. KLa increased with agitation rate and de...
Oxidation of phenolic compounds by the bifunctional catalase-phenol oxidase (CATPO) from Scytalidium thermophilum Avci, Gulden Koclar; Çoruh, Nursen; Bolukbasi, Ufuk; Ogel, Zumrut B. (Springer Science and Business Media LLC, 2013-01-01) The thermophilic fungus Scytalidium thermophilum produces a novel bifunctional catalase with an additional phenol oxidase activity (CATPO); however, its phenol oxidation spectrum is not known. Here, 14 phenolic compounds were selected as substrates, among which (+)-catechin, catechol, caffeic acid, and chlorogenic acid yielded distinct oxidation products examined by reversed-phase HPLC chromatography method. Characterization of the products by LC-ESI/MS and UV-vis spectroscopy suggests the formation of dime...
Selective oxidation and reduction reactions with cofactor regeneration mediated by galactitol-, lactate-, and formate dehydrogenases immobilized on magnetic nanoparticles Demir, Ayhan Sıtkı; Talpur, Farah N.; Sopaci, S. Betul; Kohring, Gert-W; ÇELİK, AYHAN (Elsevier BV, 2011-04-10) Rapid immobilization with the one-pot purification of galactitol dehydrogenase (GatDH) and formate dehydrogenase (FDH) is achieved by using iminodiacetic acid (IDA) with chelated Co2+ modified magnetic nanoparticles as a carrier. Lactate dehydrogenase (LDH) from recombinant Escherichia coli and FDH commencing Candida methylica were used as an auxiliary enzyme for the regeneration of NADH/NAD(+) with a representative synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol....

Citation Formats

H. GEÇKİL and P. Çalık, “Biotech in Turkey,” Biotechnology Journal, pp. 951–951, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51134.