Mixed culture growth kinetics of Streptococcus thermophilus and Lactobacillus bulgaricus

1990-2
Dik, Tunay
Özilgen, Mustafa
A simple microbiological technique was used to differentially enumerate growth of Streptococcus thermophilus and Lactobacillus bulgaricus in a mixed culture. The growth of the microorganisms in the mixed culture was satisfactorily simulated with a set of modified logistic equations. This simple model was valid for various initial biomass concentrations and their ratios. It did not need substrate or product data for simulation of biomass growth, which may simplify the calculations in fermenter design. It was shown that our model may also be regarded as a special case of a common mixed culture model: Volterra's competition analysis.
Enzyme and Microbial Technology

Suggestions

Protein-based complex medium design for recombinant serine alkaline protease production
Çalık, Pınar; Telli, IE; Oktar, C; Ozdemir, E (Elsevier BV, 2003-12-02)
This work reports on the design of a complex medium based on simple and complex carbon sources, i.e. glucose, sucrose, molasses, and defatted-soybean, and simple and complex nitrogen sources, i.e. (NH4)(2)HPO4, casein, and defatted-soybean, for serine alkaline protease (SAP) production by recombinant Bacillus subtilis carrying pHV1431::subC gene. SAP activity was obtained as 3050 U cm(-3) with the initial defatted-soybean concentration C-soybean(o) = 20 kg m(-3) and initial glucose concentration C-G(o) = 8 ...
Overexpression of serine alkaline protease encoding gene in Bacillus species: performance analyses
Çalık, Pınar; Ozdamar, TH (Elsevier BV, 2003-12-02)
Bacillus species carrying subC gene encoding serine alkaline protease (SAP) enzyme were developed in order to increase the yield and selectivity in the bioprocess for SAP production. For this aim, subC gene was cloned into pHV1431 Escherichia coli-Bacillus shuttle vector, and transferred into nine host Bacillus species, i.e. B. alvei, B. amyloliquefaciens, B. badius, B. cereus, B. coagulans, B. firmus, B. licheniformis, B. sphaericus and B. subtilis. The influence of the host Bacillus species on SAP product...
SURVIVAL KINETICS OF LACTIC-ACID STARTER CULTURES DURING AND AFTER FREEZE-DRYING
BOZOGLU, F; OZILGEN, M; BAKIR, U (Elsevier BV, 1987-09-01)
Survival kinetics of lactic acid starter cultures were modeled considering the microorganism and external medium interfacial area as the critical factors determining the resistance of the microorganisms to freeze-drying. Surviving fraction of the microorganisms increased with the increasing biomass concentration during freeze-drying, and this is attributed to the mutual shielding effect of the microorganisms against the severe conditions of the external medium. Survival of the microorganisms over the storag...
Human growth hormone-specific aptamer identification using improved oligonucleotide ligand evolution method
Çalık, Pınar; Ozdamar, Tuncer H. (Elsevier BV, 2010-01-01)
LETEG is a method developed and used for the separation and purification of proteins employing a single-step ligand (aptamers) evolution in which aptamers are eluted with an increasing temperature gradient. Using recombinant human growth hormone (rhGH) as the test purification target, and after avoiding cross reactions of aptamers with Bacillus subtilis extracellular proteins by negative SELEX, the effects of time and pH on aptamer binding to rhGH were investigated. The highest binding efficiency of aptamer...
A rapid and simple method for staining of the crystal protein of bacillus thuringiensis
Sharif, Fade A.; Alaeddinoglu, Naif Gürdal (Springer Science and Business Media LLC, 1988-6)
A rapid and simple method of staining for the crystal protein (δ-endotoxin or parasporal body) ofBacillus thuringiensis has been developed. Changes in colonial morphology were observed when cells lost their ability to form crystal protein or both crystal protein and spore.
Citation Formats
T. Dik and M. Özilgen, “Mixed culture growth kinetics of Streptococcus thermophilus and Lactobacillus bulgaricus,” Enzyme and Microbial Technology, pp. 138–140, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51440.