Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3

Kriechbaum, Maren
Heilmann, H. J.
Wientjes, F.
Hahn, M.
Jany, Klaus Dieter
Gassen, H. G.
Sharif, Fade A.
Alaeddinoglu, G.
A cDNA library from Aspergillus niger strain NRRL‐3 enriched in sequences encoding the glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12 000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5′‐ and a longer 3′‐noncoding region. The deduced protein sequence was verified by partial peptide sequencing.


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Within the heterochromatin of budding yeast, RNA polymerase II (RNAPII) transcription is repressed by the Sir2 deacetylase. Although heterochromatic silencing is generally thought to be due to limited accessibility of the underlying DNA, there are several reports of RNAPII and basal transcription factors within silenced regions. Analysis of the rDNA array revealed cryptic RNAPII transcription within the "non-transcribed" spacer region. These transcripts are terminated by the Nrd1 /Sen1 complex and degraded ...
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To determine the role of hydrophobic interactions in the dimer interface of citrate synthase (CS) from Thermoplasma (Tp) acidophilum in thermostabilization, we have used site-directed mutagenesis to replace Gly 196 by Val on the helix L of the subunit interface. Recombinant wild-type and Gly 196 mutant TpCS enzymes were largely identical in terms of substrate specificities (K-m for oxaloacetate and acetyl CoA). However, the mutation not only reduced catalytic activity (about 10-fold) (i.e., V-max, K-cat and...
Citation Formats
M. Kriechbaum et al., “Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3,” FEBS Letters, pp. 63–66, 1989, Accessed: 00, 2020. [Online]. Available: