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Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes
Date
2005-12-15
Author
Yildiz, HB
Kiralp, S
Toppare, Levent Kamil
Yagci, Y
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). Immobilization of enzyme was carried out by the entrapment in conducting polymers during electrochemical polymerization of pyrrole on the copolymer electrodes. Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities were determined for immobilized enzyme. The amount of glucose in orange juices of Turkey was investigated by using enzyme electrodes.
Subject Keywords
Biochemistry
,
Molecular Biology
,
Structural Biology
,
General Medicine
URI
https://hdl.handle.net/11511/36841
Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI
https://doi.org/10.1016/j.ijbiomac.2005.10.004
Collections
Department of Chemistry, Article
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Polyphenol oxidase (PPO) was immobilized in copolymers of thiophene functionalized menthyl monomer (MM) with pyrrole. Immobilization of enzyme was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole. Maximum reaction rates. Michaelis-Menten constants and temperature, pH and operational stabilities of enzyme electrodes were investigated. Total amount of phenolic compounds in red wines of Turkey were analyzed by using these electrodes.
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The structural and functional differences between wild type (WT) outer membrane protein G and its two mutants are investigated with Fourier transform infrared spectroscopy. Both mutants have a long extension to the primary sequence to increase the number of beta-strands from 14 (wild type) to 16 in an attempt to enlarge the pore diameter. The comparison among proteins is made in terms of pH-dependent conformational changes and thermal stability. Results show that all proteins respond to pH change but at dif...
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H. Yildiz, S. Kiralp, L. K. Toppare, and Y. Yagci, “Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes,”
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
, pp. 174–178, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36841.