Bulduklu, Yagmur
Akkaya, Mahinur
The RADIATION SENSITIVE23 (RAD23) transfers ubiquitylated substrates to proteasome and has a role in protein degradation. In our previous proteomics study, RAD23 protein was found to be over-expressed at 12 hours post inoculation (hpi) in compatible interaction (Pallas-03 inoculated with Blumeria graminis f.sp. hordei (Bgh) race 103); however it was not detectable in incompatible interaction (Pallas-01 inoculated with Bgh-103). Thus, to assess its role in barley powdery mildew resistance, the response to the virulent and avirulent pathogen races was studied on the RAD23 silenced barley seedlings using Barley Stripe Mosaic Virus (BSMV). Control plants (BSMV:00) were compared with the RAD23 silenced plants (BSMV :HvRAD23) after they are inoculated with both Bgh-103 (virulent) and Bgh-95 (avirulent) pathogen races. Hyphal growth levels were analyzed at 3 and 5 days post inoculation (dpi). HvRAD23 was found to be silenced on average 17.3% in three biological replicates. Despite the low silencing level, the change in hyphal growth was statistically significant. The primary, secondary and longest hyphae lengths were found to be decreased 21, 22 and 16%, respectively. This result together with the proteomics data suggested the idea that RAD23 protein is a negative regulator of powdery mildew resistance in barley.


Molecular cloning, characterization, and expression analysis of a gene encoding a Ran binding protein (RanBP) in Cucumis melo L.
Baloglu, Mehmet Cengiz; Zakharov, Florence Negre; Öktem, Hüseyin Avni; Yücel, Ayşe Meral (2011-01-01)
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP w...
CpG oligodeoxynucleotides protect normal and SIV-infected macaques from Leishmania infection.
Verthelyi, D; Gürsel, Mayda; Kenney, RT; Lifson, JD; Liu, S; Mican, J; Klinman, DM (The American Association of Immunologists, 2003-05-01)
Oligodeoxynucleotides containing CpG motifs (CpG ODNs) mimic microbial DNA and activate effectors of the innate immune response, which limits the spread of pathogens and promotes an adaptive immune response. CpG ODNs have been shown to protect mice from infection with intracellular pathogens. Unfortunately, CpG motifs that optimally stimulate humans are only weakly active in mice, mandating the use of nonhuman primates to monitor the activity and safety of "human" CpG ODNs in vivo. This study demonstrates t...
Analysis of protein-protein interaction networks using random walks
Can, Tolga; Singh, Ambuj K. (2005-08-21)
Genome wide protein networks have become reality in recent years due to high throughput methods for detecting protein interactions. Recent studies show that a networked representation of proteins provides a more accurate model of biological systems and processes compared to conventional pair-wise analyses. Complementary to the availability of protein networks, various graph analysis techniques have been proposed to mine these networks for pathway discovery, function assignment, and prediction of complex mem...
CpG RNA: identification of novel single-stranded RNA that stimulates human CD14+CD11c+ monocytes.
Sugiyama, T; Gürsel, Mayda; Takeshita, F; Coban, C; Conover, J; Kaisho, T; Akira, S; Klinman, DM; Ishii, KJ (The American Association of Immunologists, 2005-02-15)
Synthetic immunostimulatory nucleic acids such as CpG DNA are being harnessed therapeutically as vaccine adjuvants, anticancer or antiallergic agents. Efforts to identify nucleic acid-based agents capable of more specifically modulating the immune system are being developed. The current study identifies a novel class of single-stranded oligoribonucleotides (ORN) containing unmethylated CpG motifs and a poly(G) run at the 3 end (CpG ORN) that directly stimulate human CD14 CD11c monocytes but not dendritic ...
Degradation of poly(bisphenol-a-epichlorohydrin) by gamma irradiation
Ozdemir, Tonguc; Usanmaz, Ali (Elsevier BV, 2008-06-01)
In this study, radiation stability of poly(bisphenol-a-epichloroliydrin) (PBEH) was studied via gamma irradiations at two different dose rates of 1540 and 82.8 Gy/h, in order to understand the possible use of PBEH in radioactive waste management as a solidifying agent. The total dose of irradiation was up to 2150kGy. Degradation nature was tested by studying the changes in mechanical and thermal properties with the change of dose rate and total dose of irradiation. Tensile strength at yield was increased fi...
Citation Formats
Y. Bulduklu, F. ERSOY, and M. Akkaya, “RAD23 AS A NEGATIVE REGULATOR OF BARLEY POWDERY MILDEW DISEASE RESISTANCE,” FRESENIUS ENVIRONMENTAL BULLETIN, pp. 6239–6244, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54350.