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Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients
Date
2000-02-01
Author
Eren, E
Aytac, U
Tetik, E
Akman, O
Kansu, E
Gündüz, Ufuk
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph), However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment. Am. J. Hematol, 63:85-89, 2000, (C) 2000 Wiley-Liss, Inc.
Subject Keywords
RT-PCR
,
Interferon-alpha
,
bcr/abl
,
Ph
,
CML
URI
https://hdl.handle.net/11511/54366
Journal
AMERICAN JOURNAL OF HEMATOLOGY
Collections
Graduate School of Natural and Applied Sciences, Article