Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Date

2000-02-01

Author

Eren, E
Aytac, U
Tetik, E
Akman, O
Kansu, E
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

32
views

0
downloads

Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph), However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment. Am. J. Hematol, 63:85-89, 2000, (C) 2000 Wiley-Liss, Inc.

Subject Keywords

RT-PCR, Interferon-alpha, bcr/abl, Ph, CML

URI

https://hdl.handle.net/11511/54366

Journal

AMERICAN JOURNAL OF HEMATOLOGY

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

ANALYSIS OF PLASMIDS MEDIATING ANT (2'') AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE Kocabıyık, Semra (Elsevier BV, 1989-07-01) The genetics of ANT(2″) and AAC(3)-II mediated gentamicin resistance in Gram-negative bacteria obtained from Hacettepe University Hospital in Turkey, was studied. The plasmid DNA analyzed by agarose gel electrophoresis revealed a heterogeneous plasmid population. Conjugal transfer appeared to be an efficient mechanism for the dissemination of resistance to gentamicin/tobramycin/kanamycin/streptomycin/chloramphenicol/cephalothin, which was the common resistance pattern in the population. Plasmids of 46, 60, ...
Association of-174G/C interleukin-6 gene polymorphism with the risk of chronic lymphocytic, chronic myelogenous and acute myelogenous leukemias in Turkish patients Mutlu, Pelin; Elci, Pinar; Yildirim, Murat; Cetin, A. Turker; Avcu, Ferit (2014-07-01) Purpose: The purpose of this study was to evaluate the relationship between -174G/C interleukin-6 (IL-6) gene promoter polymorphism and susceptibility to chronic lymphocytic (CLL), chronic myelogenous (CML) and acute myelogenous leukemia (AML) in Turkish patients.
Identification of polymorphisms on the MDR1 gene among Turkish population and their effects on multidrug resistance in acute leukemia patients Kaya, P; Gündüz, Ufuk; Arpaci, F; Ural, AU; Guran, S (2005-09-01) Multidrug-resistance (MDR) phenotype is a serious limitation to the effective chemotherapeutic treatment of many cancer types, including leukemia. One of the most important proteins, the over-expression of which is responsible for the multidrug-resistance phenotype in many cancer types, is P-glycoprotein. This protein is the product of the MDR1 gene. In previous studies, single-nucleotide polymorphisms (SNPs) C3435T, G2677T, and T-129C in the MDR1 gene were shown to be correlated with lower P-glycoprotein e...
Characterization and Evaluation of Triamcinolone, Raloxifene, and Their Dual-Loaded Microspheres as Prospective Local Treatment System in Rheumatic Rat Joints Ocal, Yigit; Kurum, Baris; Karahan, Siyami; Tezcaner, Ayşen; Ozen, Seza; Keskin, Dilek (Elsevier BV, 2014-8) In this study, injectable microspheres were developed for the local treatment of joint degeneration in rheumatoid arthritis (RA). Microspheres loaded with triamcinolone (TA), a corticosteroid drug, and/or raloxifene (Ral), a cartilage regenerative drug, were prepared with a biodegradable and biocompatible polymer, polycaprolactone (PCL). Microspheres were optimized for particle size, structural properties, drug release, and loading properties. In vitro release of Ral was very slow because of the low solubil...
Characterization of Novel O-Glycans Isolated from Tear and Saliva of Ocular Rosacea Patients Özcan Kabasakal, Süreyya; Vieira, Ana C.; Park, Gun Wook; Kim, Jae Han; Mannis, Mark J.; Lebrilla, Carlito B. (American Chemical Society (ACS), 2013-03-01) O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients In this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the ...

Citation Formats

E. Eren, U. Aytac, E. Tetik, O. Akman, E. Kansu, and U. Gündüz, “Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients,” AMERICAN JOURNAL OF HEMATOLOGY, pp. 85–89, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54366.