ANALYSIS OF PLASMIDS MEDIATING ANT (2'') AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE

Date

1989-07-01

Author

Kocabıyık, Semra

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

218
views

0
downloads

The genetics of ANT(2″) and AAC(3)-II mediated gentamicin resistance in Gram-negative bacteria obtained from Hacettepe University Hospital in Turkey, was studied. The plasmid DNA analyzed by agarose gel electrophoresis revealed a heterogeneous plasmid population. Conjugal transfer appeared to be an efficient mechanism for the dissemination of resistance to gentamicin/tobramycin/kanamycin/streptomycin/chloramphenicol/cephalothin, which was the common resistance pattern in the population. Plasmids of 46, 60, 70 and 80 Mdal in size were found to be conjugative. Among these a 70 Mdal plasmid was isolated more frequently; it was harboured by the strains exhibiting either of the enzyme profiles, ANT(2″) or AAC(3)-II. Like the substrate profiles of the donors, their enzymatic activities were also maintained in the respective transconjugants. Based on the donor and transconjugant resistance markers, agarose gel electrophoresis and transfer properties, ANT(2″) and AAC(3)-II dependent gentamicin resistance was found to be plasmid-linked. However, the evidence of a possible chromosomal location for the gentamicin resistance suggested that the resistance might well be of a transposable nature.

Subject Keywords

Immunology

URI

https://hdl.handle.net/11511/37553

Journal

ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES

DOI

https://doi.org/10.1016/s0934-8840(89)80075-1

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Effect of suppressive DNA on CpG-induced immune activation. Yamada, H; Gursel, I; Takeshita, F; Conover, J; Ishii, KJ; Gürsel, Mayda; Takeshita, S; Klinman, DM (The American Association of Immunologists, 2002-11-15) Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the CpG DNA or adding inhibitory/suppressive motifs. Suppression is dominant over stimulation and is specific for CpG-induced immune responses (having no effect on LPS- or Con A-induced activation). Individual cells noncompetitively internalize both stimulatory and suppressive ODN. Studies using ODN composed of both stimula...
CpG oligodeoxynucleotides protect normal and SIV-infected macaques from Leishmania infection. Verthelyi, D; Gürsel, Mayda; Kenney, RT; Lifson, JD; Liu, S; Mican, J; Klinman, DM (The American Association of Immunologists, 2003-05-01) Oligodeoxynucleotides containing CpG motifs (CpG ODNs) mimic microbial DNA and activate effectors of the innate immune response, which limits the spread of pathogens and promotes an adaptive immune response. CpG ODNs have been shown to protect mice from infection with intracellular pathogens. Unfortunately, CpG motifs that optimally stimulate humans are only weakly active in mice, mandating the use of nonhuman primates to monitor the activity and safety of "human" CpG ODNs in vivo. This study demonstrates t...
Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.) Cicerali, Işın Nur; Yücel, Ayşe Meral; Department of Biotechnology (2004) In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase...
Investigation of the role of a candidate effector of wheat stripe rust pathogen in plant immunity Dagvadorj, Bayantes; Akkaya, Mahinur S.; Hakkı, Erdoğan Eşref; Department of Biotechnology (2016) The advanced plant molecular biology and plant biotechnology tools were employed in this thesis, in order to understand the role of a candidate effector, PstSCR1, of Puccinia striiformis f. sp. tritici (Pst) causing yellow rust disease in wheat. The homologues proteins of PstSCR1 were found to be only conserved in Pst, and in its closest relative, Puccinia graminis f. sp. tritici (Pgt). When PstSCR1 was expressed in Nicotiana benthamiana with its signal peptide (SP), it provoked the plant defense, whereas n...
CpG RNA: identification of novel single-stranded RNA that stimulates human CD14+CD11c+ monocytes. Sugiyama, T; Gürsel, Mayda; Takeshita, F; Coban, C; Conover, J; Kaisho, T; Akira, S; Klinman, DM; Ishii, KJ (The American Association of Immunologists, 2005-02-15) Synthetic immunostimulatory nucleic acids such as CpG DNA are being harnessed therapeutically as vaccine adjuvants, anticancer or antiallergic agents. Efforts to identify nucleic acid-based agents capable of more specifically modulating the immune system are being developed. The current study identifies a novel class of single-stranded oligoribonucleotides (ORN) containing unmethylated CpG motifs and a poly(G) run at the 3 end (CpG ORN) that directly stimulate human CD14 CD11c monocytes but not dendritic ...

Citation Formats

S. Kocabıyık, “ANALYSIS OF PLASMIDS MEDIATING ANT (2″) AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE,” ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES, pp. 214–221, 1989, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37553.