Xylanase and itaconic acid production by Aspergillus terreus NRRL 1960 within a biorefinery concept

Kocabas, Aytac
Ögel, Zümrüt Begüm
Bakir, Ufuk
Production of two industrially important products, xylanase and itaconic acid (IA), by Aspergillus terreus NRRL 1960 from agricultural residues was investigated within a biorefinery concept. Biological pretreatment was applied to lignocellulosic materials by using A. terreus, which produced xylanase while growing on agricultural residues. For IA production, already grown cells were transferred into a new medium. The first step provided not only the pretreatment of lignocellulosic material in order to be used as feedstock but also production of xylanase. For this purpose, cotton stalk, sunflower stalk and corn cob were used as carbon sources as lignocellulosic material. Among them, the highest xylanase production was obtained on corn cob. By application of two-step fermentation, about 70 IU/mL xylanase and 18 g/L IA production levels were achieved. This study shows the stepwise usage potential of the microorganism as a tool in a biorefinery concept.


Co-production of xylanase and itaconic acid by aspergillus terreus nrrl 1960 on agricultural biomass and biochemical characterization of xylanase
Kocabaş, Aytaç; Bakır, Ufuk; Department of Biotechnology (2010)
Production of xylanase and itaconic acid (IA) from Aspergillus terreus NRRL 1960 from agricultural residues was investigated in this study. Two different media were tested and the medium having itaconic acid inducing capacity was chosen for further studies due to its high xylanase and IA production capacity. The best xylan concentration was found as 2% (w/v). Addition of commercial xylanase to production culture resulted in higher initial simple sugar concentration which increased IA production slightly but...
Protein-based complex medium design for recombinant serine alkaline protease production
Çalık, Pınar; Telli, IE; Oktar, C; Ozdemir, E (Elsevier BV, 2003-12-02)
This work reports on the design of a complex medium based on simple and complex carbon sources, i.e. glucose, sucrose, molasses, and defatted-soybean, and simple and complex nitrogen sources, i.e. (NH4)(2)HPO4, casein, and defatted-soybean, for serine alkaline protease (SAP) production by recombinant Bacillus subtilis carrying pHV1431::subC gene. SAP activity was obtained as 3050 U cm(-3) with the initial defatted-soybean concentration C-soybean(o) = 20 kg m(-3) and initial glucose concentration C-G(o) = 8 ...
Bacilysin biosynthesis by a partially-purified enzyme fraction from Bacillus subtilis
Yazgan, A; Özcengiz, Gülay; Ozcengiz, E; Kilinc, K; Marahiel, MA; Alaeddinoglu, NG (Elsevier BV, 2001-10-04)
Biosynthesis of dipeptide antibiotic bacilysin by a partially purified enzyme prepared from Bacillus subtilis PY79 was studied. Cell material was desintegrated by treatment with lysozyme and sonication and the extract was subjected to ammonium sulfate fractionation. Bacilysin-synthesizing enzyme activity was precipitated between 40% to 70% ammonium sulfate saturation. In vitro enzymatical synthesis of bacilysin was confirmed by performing thin layer chromatographic comparison of the antibiotic formed with t...
Avicel-adsorbable endoglucanase production by the thermophilic fungus Scytalidium thermophilum type culture Torula thermophila
Arifoglu, N; Ögel, Zümrüt Begüm (Elsevier BV, 2000-11-01)
Scytalidium thermophilum type culture Torula thermophila was isolated from mushroom compost and the total cellulase, endoglucanase, Avicel-adsorbable endoglucanase activities, as well as the fungal biomass generation and cellulose utilisation were analyzed in shake flask cultures with Avicel (microcrystalline cellulose) as the carbon source. Results were compared with an industrial strain of Scyalidium thermophilum type culture Humicola insolens. The pH and temperature optima for endoglucanase activities du...
TUKENMEZ, I; OZILGEN, M; BICER, A (Elsevier BV, 1991-11-01)
Rumen microorganisms were used in a batch fermentor to investigate whether they could be used to produce acetic acid, propionic acid, butyric acid, and methane from cotton fibers. The kinetics of product formation were studied with the initial medium pH values of 6.0, 7.0, and 8.0. Propionic acid, butyric acid, and methane production were growth associated; acetic acid production was found to be nongrowth associated. The largest amounts of bacteria and protozoa were attained with the initial pH of 7.0. T...
Citation Formats
A. Kocabas, Z. B. Ögel, and U. Bakir, “Xylanase and itaconic acid production by Aspergillus terreus NRRL 1960 within a biorefinery concept,” ANNALS OF MICROBIOLOGY, pp. 75–84, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56535.