Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks

2013-11-01
Haykir, Nazife Isik
Bakir, Ufuk
To enhance the enzymatic conversion of cotton stalks to glucose at high substrate loadings and subsequently to produce ethanol, biomass was subjected to ionic liquid and alkaline pretreatments. Ionic liquid, 1-ethyl-3-methylimidazolium acetate (EMIMAc), which reduced biomass crystallinity, was effective in improving the conversion of cotton stalks to glucose (82-85%) regardless of the substrate loading used during enzymatic hydrolysis. On the other hand, the glucose yield decreased from 67% to 55% with an increase in substrate loading from 3% to 15% (w/v) for the alkaline pretreated biomass. Fermentation of the glucose obtained upon enzymatic hydrolysis of the pretreated cotton stalks at the highest substrate loading (15%) resulted in ethanol yields of 74% and 46% for EMIMAc and alkaline pretreated biomass, respectively. These results highlight the advantage of EMIMAc pretreatment to maintain increased substrate loadings during enzymatic hydrolysis of lignocellulosic biomass for achieving high concentrations of products with improved yields.
INDUSTRIAL CROPS AND PRODUCTS

Suggestions

Simultaneous dissolution and uptake of nutrients in microalgal treatment of the secondarily treated digestate
Ulgudur, Nilufer; Bayramoğlu, Tuba Hande; Demirer, Goksel N. (Elsevier BV, 2019-11-01)
Anaerobic digestion processes result in large volumes of digestates which still require the development of elaborate and viable management options to improve the sustainability of these processes. Coupling of secondary anaerobic treatment with a microalgal nutrient removal process may serve benefits such as residual biogas and associated greenhouse gas capture from the digestate content, microalgal biomass build-up and its further valorization. This study investigated the applicability a microalgal nutrient...
Micromeria myrtifolia: The influence of the extracting solvents on phenolic composition and biological activity
Sarikurkcu, Cengiz; Hanine, Hafida; Sarikurkcu, Rabia Busra; Sarikurkcu, Rifat Tayyib; Amarowicz, Ryszard (Elsevier BV, 2020-03-01)
Currently, there is much interest in using the active compounds that exist in plants as natural treatments. The pharmaceutical industry is also looking for novel chemical compounds from natural sources that do not have side effects. The purpose of this study was to identify the antioxidant and enzymatic inhibition activities of different extracts (ethyl acetate, methanol and water) of Micromeria myrtifolia from Turkey. The phenolic constituents in the extracts were also identified using liquid chromatograph...
Anaerobic acidification of sugar-beet processing wastes: Effect of operational parameters
Alkaya, Emrah; Demirer, Göksel Niyazi (Elsevier BV, 2011-01-01)
The objective of this study was to maximize the hydrolysis and acidification of sugar-beet processing wastewater and beet pulp for volatile fatty acid (VFA) production through acidogenic anaerobic metabolism. Experiments were conducted to determine the optimum operational conditions (HRT, waste-mixing ratio and pH) for effective acidification in daily-fed, continuously mixed anaerobic reactors. For this purpose, reactors were operated at 35 +/- 1 degrees C with different combinations of HRT (2-4 days), wast...
Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis
Çalık, Pınar; Özdamar, Tunçer H. (2000-08-01)
The effects of oxygen transfer on the production and product distribution in serine alkaline protease (SAP) fermentation by Bacillus licheniformis and oxygen-transfer strategy in relation to the physiology of the bacilli were investigated on a defined medium with citric acid as sole carbon source in 3.5-dm(3) batch bioreactor systems. By forming a 3 x 3 matrix with the parameters air-inlet rates of Q(O)/V-R = 0.2, 0.5, 1.0 vvm, and agitation rates of N = 150, 500, 750 min(-1), the effects of oxygen transfer...
KINETIC COMPENSATION RELATIONS FOR ASCORBIC-ACID DEGRADATION AND PECTINESTERASE INACTIVATION DURING ORANGE JUICE PASTEURIZATION
ULGEN, N; OZILGEN, M (Wiley, 1991-01-01)
The thermal degradation kinetics of ascorbic acid and the inactivation kinetics of pectinesterase were studied experimentally with pH-adjusted orange juice. No simple trends were observed in variation of the kinetic parameters with pH, but both kinetic functions agreed with kinetic compensation relations. An isokinetic temperature was observed for ascorbic acid degradation at pH 2.5, 3.0, and 3.5 implying that the same degradation mechanism was valid at these pH values. A large deviation at pH 4.0 from t...
Citation Formats
N. I. Haykir and U. Bakir, “Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks,” INDUSTRIAL CROPS AND PRODUCTS, pp. 408–414, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/64795.